Cholesterol 25-hydroxylase negatively regulates porcine intestinal coronavirus replication by the production of 25-hydroxycholesterol.
25-hydroxycholesterol
Cholesterol 25-hydroxylase
Porcine epidemic diarrhea virus
Porcine transmissible gastroenteritis virus
Journal
Veterinary microbiology
ISSN: 1873-2542
Titre abrégé: Vet Microbiol
Pays: Netherlands
ID NLM: 7705469
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
27
12
2018
revised:
05
03
2019
accepted:
06
03
2019
entrez:
9
4
2019
pubmed:
9
4
2019
medline:
3
5
2019
Statut:
ppublish
Résumé
Cholesterol 25-hydroxylase (CH25H) has been shown lately to be a host restriction factor that encodes an enzyme, which catalyzes the oxidized form of cholesterol to 25-hydroxycholesterol (25HC). A series of studies have shown that 25HC activity in hosts plays a vital role in inhibiting viral infection. In this study, we explored the antiviral effect of CH25H and 25HC on porcine epidemic diarrhea virus (PEDV), which causes high mortality rates in newborn piglets with severe diarrhea, and considerable financial loss in the swine industry worldwide. Our results showed that PEDV infection downregulated the expression of CH25H in Vero cells. An overexpression and knockdown assay indicated that CH25H has significant antiviral action against PEDV, and a CH25H mutant (CH25H-M) that lacks hydroxylase activity also retains antiviral activity to a lesser extent. Furthermore, 25HC had a broad-spectrum antiviral effect against different PEDV strains by blocking viral entry. In addition, CH25H and 25HC inhibited the replication of porcine transmissible gastroenteritis virus (TGEV). Taken together, CH25H as a natural host restriction factor could inhibit PEDV and TGEV infection.
Identifiants
pubmed: 30955800
pii: S0378-1135(18)31522-0
doi: 10.1016/j.vetmic.2019.03.004
pmc: PMC7117535
pii:
doi:
Substances chimiques
Hydroxycholesterols
0
25-hydroxycholesterol
767JTD2N31
Steroid Hydroxylases
EC 1.14.-
cholesterol 25-hydroxylase
EC 1.14.99.38
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
129-138Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.
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