Transitions in cell potency during early mouse development are driven by Notch.
Cdx2
chromosomes
developmental biology
gene expression
morula
mouse
notch
preimplantation development
trophectoderm
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
08 04 2019
08 04 2019
Historique:
received:
17
10
2018
accepted:
07
04
2019
pubmed:
9
4
2019
medline:
29
2
2020
entrez:
9
4
2019
Statut:
epublish
Résumé
The Notch signalling pathway plays fundamental roles in diverse developmental processes in metazoans, where it is important in driving cell fate and directing differentiation of various cell types. However, we still have limited knowledge about the role of Notch in early preimplantation stages of mammalian development, or how it interacts with other signalling pathways active at these stages such as Hippo. By using genetic and pharmacological tools in vivo, together with image analysis of single embryos and pluripotent cell culture, we have found that Notch is active from the 4-cell stage. Transcriptomic analysis in single morula identified novel Notch targets, such as early naïve pluripotency markers or transcriptional repressors such as TLE4. Our results reveal a previously undescribed role for Notch in driving transitions during the gradual loss of potency that takes place in the early mouse embryo prior to the first lineage decisions.
Identifiants
pubmed: 30958266
doi: 10.7554/eLife.42930
pii: 42930
pmc: PMC6486152
doi:
pii:
Substances chimiques
Receptors, Notch
0
Banques de données
GEO
['GSE121979', 'GSE66390']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIH HHS
ID : NIH-P30CA008748
Pays : United States
Organisme : NIH HHS
ID : NIH-R01DK084391
Pays : United States
Organisme : Ministerio de Economía y Competitividad
ID : SVP-2013-067930
Pays : International
Organisme : Ministerio de Economía y Competitividad
ID : BFU2015-72319-EXP
Pays : International
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : Ministerio de Economía y Competitividad
ID : SEV-2015-0505
Pays : International
Organisme : NICHD NIH HHS
ID : R01 HD094868
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK084391
Pays : United States
Organisme : NIH HHS
ID : NIH-R01HD094868
Pays : United States
Organisme : Ministerio de Economía y Competitividad
ID : BFU2017-84914-P
Pays : International
Informations de copyright
© 2019, Menchero et al.
Déclaration de conflit d'intérêts
SM, IR, AL, MA, JS, MK, JA, RB, TR, AH, MM No competing interests declared
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