Identification of a molecular gating determinant within the carboxy terminal region of Ca


Journal

Molecular brain
ISSN: 1756-6606
Titre abrégé: Mol Brain
Pays: England
ID NLM: 101468876

Informations de publication

Date de publication:
08 04 2019
Historique:
received: 24 02 2019
accepted: 01 04 2019
entrez: 10 4 2019
pubmed: 10 4 2019
medline: 1 8 2019
Statut: epublish

Résumé

The physiological functions controlled by T-type channels are intrinsically dependent on their gating properties, and alteration of T-type channel activity is linked to several human disorders. Therefore, it is essential to develop a clear understanding of the structural determinants responsible for the unique gating features of T-type channels. Here, we have investigated the specific role of the carboxy terminal region by creating a series a deletion constructs expressed in tsA-201 cells and analyzing them by patch clamp electrophysiology. Our data reveal that the proximal region of the carboxy terminus contains a structural determinant essential for shaping several gating aspects of Ca

Identifiants

pubmed: 30961646
doi: 10.1186/s13041-019-0457-0
pii: 10.1186/s13041-019-0457-0
pmc: PMC6454634
doi:

Substances chimiques

CACNA1I protein, human 0
Calcium Channels, T-Type 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Pagination

34

Subventions

Organisme : CIHR
Pays : Canada

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Auteurs

Bohumila Jurkovicova-Tarabova (B)

Center of Biosciences, Institute of Molecular Physiology and Genetics, Academy of Sciences, Bratislava, Slovakia.

Leos Cmarko (L)

Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nam. 2, 16610, Prague, Czech Republic.

Renata Rehak (R)

Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, Calgary, Canada.

Gerald W Zamponi (GW)

Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, Calgary, Canada.

Lubica Lacinova (L)

Center of Biosciences, Institute of Molecular Physiology and Genetics, Academy of Sciences, Bratislava, Slovakia.

Norbert Weiss (N)

Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nam. 2, 16610, Prague, Czech Republic. weiss@uochb.cas.cz.

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Classifications MeSH