Sodium butyrate ameliorates Corynebacterium pseudotuberculosis infection in RAW264.7 macrophages and C57BL/6 mice.
Animals
Anti-Bacterial Agents
/ pharmacology
Antimicrobial Cationic Peptides
/ genetics
Bacterial Load
/ drug effects
Butyric Acid
/ pharmacology
Corynebacterium Infections
/ microbiology
Corynebacterium pseudotuberculosis
/ drug effects
Disease Models, Animal
Drug Combinations
Female
Gene Expression Regulation, Bacterial
/ drug effects
Genes, Bacterial
/ genetics
Macrophages
/ drug effects
Mice
Mice, Inbred C57BL
RAW 264.7 Cells
/ drug effects
Spleen
/ microbiology
Cathelicidins
Antimicrobial peptides
Corynebacterium pseudotuberculosis
Mice
RAW264.7 macrophages
Sodium butyrate
Virulence genes
Journal
Microbial pathogenesis
ISSN: 1096-1208
Titre abrégé: Microb Pathog
Pays: England
ID NLM: 8606191
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
13
02
2019
revised:
23
03
2019
accepted:
05
04
2019
pubmed:
10
4
2019
medline:
30
7
2019
entrez:
10
4
2019
Statut:
ppublish
Résumé
Corynebacterium pseudotuberculosis (CP) infection in livestock has become highly difficult to control. To decrease the incidence of CP infection, the supplementation of feed with non-antibiotic antibacterial substances is a potential approach. The aim of this study was to assess the effects of sodium butyrate (NaB), a potential alternative to antibiotics, on CP infection in RAW264.7 macrophages and C57BL/6 mice. Our data showed that NaB (2 mM) significantly ameliorated CPinfection in RAW264.7 macrophages and decreased the bacterial load in the spleens of infected mice. By real-time PCR, we found that NaB induced significant decreases in zinc-dependent superoxide dismutase (sodC) and tip protein C (spaC) expression in CP from infected-RAW264.7 cells and in phospholipase D (pld) and spaC expression in CP from the spleens of infected mice. NaB treatment significantly up-regulated cathelicidin-related antimicrobial peptide (cramp) expression in spleens of mice infected with CP. Furthermore, NaB alleviated histopathological changes in spleens of CP-infected mice. In conclusion, NaB ameliorated CP infection in RAW264.7 macrophages and C57BL/6 mice, and these effects may be related to the modulation of sodC, spaC, pld, and cramp expression.
Identifiants
pubmed: 30965088
pii: S0882-4010(19)30277-3
doi: 10.1016/j.micpath.2019.04.008
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Antimicrobial Cationic Peptides
0
Drug Combinations
0
Butyric Acid
107-92-6
Cathelicidins
0
Types de publication
Journal Article
Langues
eng
Pagination
144-149Informations de copyright
Copyright © 2019. Published by Elsevier Ltd.