Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy.
Animals
Astrocytes
/ metabolism
Cerebral Cortex
/ cytology
Color
Dependovirus
Female
Genetic Vectors
/ administration & dosage
HEK293 Cells
Humans
Imaging, Three-Dimensional
/ methods
Luminescent Proteins
/ chemistry
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microscopy, Fluorescence, Multiphoton
/ methods
Models, Animal
Nestin
/ genetics
Neuroanatomical Tract-Tracing Techniques
/ methods
Neuroimaging
/ methods
Parvovirinae
/ genetics
Pyramidal Cells
/ metabolism
Transfection
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
10 04 2019
10 04 2019
Historique:
received:
01
03
2018
accepted:
12
03
2019
entrez:
12
4
2019
pubmed:
12
4
2019
medline:
30
4
2019
Statut:
epublish
Résumé
Large-scale microscopy approaches are transforming brain imaging, but currently lack efficient multicolor contrast modalities. We introduce chromatic multiphoton serial (ChroMS) microscopy, a method integrating one-shot multicolor multiphoton excitation through wavelength mixing and serial block-face image acquisition. This approach provides organ-scale micrometric imaging of spectrally distinct fluorescent proteins and label-free nonlinear signals with constant micrometer-scale resolution and sub-micron channel registration over the entire imaged volume. We demonstrate tridimensional (3D) multicolor imaging over several cubic millimeters as well as brain-wide serial 2D multichannel imaging. We illustrate the strengths of this method through color-based 3D analysis of astrocyte morphology and contacts in the mouse cerebral cortex, tracing of individual pyramidal neurons within densely Brainbow-labeled tissue, and multiplexed whole-brain mapping of axonal projections labeled with spectrally distinct tracers. ChroMS will be an asset for multiscale and system-level studies in neuroscience and beyond.
Identifiants
pubmed: 30971684
doi: 10.1038/s41467-019-09552-9
pii: 10.1038/s41467-019-09552-9
pmc: PMC6458155
doi:
Substances chimiques
Luminescent Proteins
0
Nes protein, mouse
0
Nestin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1662Commentaires et corrections
Type : ErratumIn
Type : CommentIn
Références
Opt Express. 2008 Nov 24;16(24):19396-409
pubmed: 19030027
Nat Methods. 2015 Jun;12(6):547-52
pubmed: 25915122
Nat Methods. 2012 Jul 08;9(8):815-8
pubmed: 22772730
J Biomed Opt. 2007 Jan-Feb;12(1):014015
pubmed: 17343490
Elife. 2016 Jan 20;5:e10566
pubmed: 26796534
Neuron. 2014 Feb 5;81(3):505-20
pubmed: 24507188
Elife. 2018 Nov 20;7:
pubmed: 30454553
J Neurosci. 2013 Mar 27;33(13):5718-27
pubmed: 23536085
Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):E2714-23
pubmed: 23812756
Nature. 2012 Apr 25;484(7395):479-84
pubmed: 22538609
Nature. 2007 Nov 1;450(7166):56-62
pubmed: 17972876
Biomed Opt Express. 2014 Apr 18;5(5):1588-609
pubmed: 24877017
Bioinformatics. 2009 Jun 1;25(11):1463-5
pubmed: 19346324
Cell. 2008 Feb 8;132(3):487-98
pubmed: 18267078
Cell. 2010 Oct 1;143(1):134-44
pubmed: 20887898
Science. 2010 Dec 3;330(6009):1404-8
pubmed: 21051596
Nat Methods. 2013 May 5;10(6):540-7
pubmed: 23817127
Neuroimage. 2013 Jul 1;74:87-98
pubmed: 23416252
PLoS One. 2008 Apr 16;3(4):e2005
pubmed: 18414675
Nat Commun. 2018 Sep 3;9(1):3549
pubmed: 30177709
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Science. 2002 May 3;296(5569):913-6
pubmed: 11988576
Biophys J. 2011 Mar 2;100(5):1362-71
pubmed: 21354410
Int J Dev Neurosci. 2004 Apr;22(2):73-86
pubmed: 15036382
Nat Neurosci. 2017 Aug;20(8):1172-1179
pubmed: 28671695
Nat Methods. 2012 Jan 15;9(3):255-8
pubmed: 22245809
J Comp Neurol. 2016 Dec 1;524(17):3561-3576
pubmed: 27072916
J Neurosci. 2007 Jun 13;27(24):6473-7
pubmed: 17567808
Nat Methods. 2017 Aug;14(8):775-781
pubmed: 28775673
Cell. 2014 Apr 24;157(3):726-39
pubmed: 24746791
Proc Natl Acad Sci U S A. 2011 Apr 12;108(15):5970-5
pubmed: 21444784
Small. 2017 Nov;13(42):
pubmed: 28926684
Nat Neurosci. 2017 Jun;20(6):793-803
pubmed: 28414331
Nat Methods. 2016 Dec;13(12):993-996
pubmed: 27798610
J Neurosci. 2007 Oct 17;27(42):11334-42
pubmed: 17942728
Cell Rep. 2016 Apr 26;15(4):692-699
pubmed: 27149846
Neuron. 2017 May 3;94(3):517-533.e3
pubmed: 28472654
Nat Biotechnol. 2004 Apr;22(4):445-9
pubmed: 14990965
Nat Genet. 1999 Sep;23(1):99-103
pubmed: 10471508
Neuron. 2014 Sep 17;83(6):1431-43
pubmed: 25175879
Nat Biotechnol. 2004 Dec;22(12):1567-72
pubmed: 15558047
Nat Neurosci. 2016 Aug;19(8):1100-14
pubmed: 27322419
Cell. 2014 Jun 19;157(7):1724-34
pubmed: 24949979
Neuron. 2016 Sep 21;91(6):1228-1243
pubmed: 27618674
Nat Commun. 2018 Apr 24;9(1):1623
pubmed: 29691400
Science. 2015 Nov 27;350(6264):aaa8870
pubmed: 26612955
Cell Rep. 2015 Sep 8;12(10):1575-83
pubmed: 26321636
Nature. 2014 Apr 10;508(7495):207-14
pubmed: 24695228
Cell. 2005 May 6;121(3):479-92
pubmed: 15882628
Nat Biotechnol. 2002 Jan;20(1):83-7
pubmed: 11753367
Nat Commun. 2016 Jul 04;7:12142
pubmed: 27374071
Genetics. 2015 Feb;199(2):293-306
pubmed: 25657347
Proc Natl Acad Sci U S A. 2015 Oct 27;112(43):E5873-82
pubmed: 26450880
Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1531-6
pubmed: 21205896
Nat Commun. 2012 Mar 20;3:751
pubmed: 22434194
Cell. 2014 Nov 6;159(4):896-910
pubmed: 25417164
Neuron. 2013 Dec 18;80(6):1368-83
pubmed: 24360541
Cell. 2017 Aug 10;170(4):800-814.e18
pubmed: 28802047
Nature. 2012 Mar 28;484(7394):376-80
pubmed: 22456708
Nat Methods. 2013 Jun;10(6):508-13
pubmed: 23722210
Dev Cell. 2016 Nov 7;39(3):289-301
pubmed: 27923766
Sci Rep. 2016 Feb 02;6:20072
pubmed: 26830143
Development. 2014 May;141(9):1971-80
pubmed: 24718991
Sci Rep. 2016 Sep 22;6:33896
pubmed: 27654510
Nat Methods. 2006 Jan;3(1):47-53
pubmed: 16369553
Curr Biol. 2013 Jan 7;23(1):21-31
pubmed: 23177476