The potato cyst nematode effector RHA1B is a ubiquitin ligase and uses two distinct mechanisms to suppress plant immune signaling.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
31
07
2018
accepted:
20
03
2019
entrez:
13
4
2019
pubmed:
13
4
2019
medline:
18
5
2019
Statut:
epublish
Résumé
Plant pathogens, such as bacteria, fungi, oomycetes and nematodes, rely on wide range of virulent effectors delivered into host cells to suppress plant immunity. Although phytobacterial effectors have been intensively investigated, little is known about the function of effectors of plant-parasitic nematodes, such as Globodera pallida, a cyst nematode responsible for vast losses in the potato and tomato industries. Here, we demonstrate using in vivo and in vitro ubiquitination assays the potato cyst nematode (Globodera pallida) effector RHA1B is an E3 ubiquitin ligase that employs multiple host plant E2 ubiquitin conjugation enzymes to catalyze ubiquitination. RHA1B was able to suppress effector-triggered immunity (ETI), as manifested by suppression of hypersensitive response (HR) mediated by a broad range of nucleotide-binding leucine-rich repeat (NB-LRR) immune receptors, presumably via E3-dependent degradation of the NB-LRR receptors. RHA1B also blocked the flg22-triggered expression of Acre31 and WRKY22, marker genes of pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI), but this did not require the E3 activity of RHA1B. Moreover, transgenic potato overexpressing the RHA1B transgene exhibited enhanced susceptibility to G. pallida. Thus, our data suggest RHA1B facilitates nematode parasitism not only by triggering degradation of NB-LRR immune receptors to block ETI signaling but also by suppressing PTI signaling via an as yet unknown E3-independent mechanism.
Identifiants
pubmed: 30978251
doi: 10.1371/journal.ppat.1007720
pii: PPATHOGENS-D-18-01540
pmc: PMC6461251
doi:
Substances chimiques
Plant Proteins
0
Ubiquitin
0
Ubiquitin-Protein Ligases
EC 2.3.2.27
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1007720Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Annu Rev Biochem. 2001;70:503-33
pubmed: 11395416
Proc Natl Acad Sci U S A. 2001 Oct 9;98(21):12295-300
pubmed: 11593047
Plant J. 2002 Oct;32(2):195-204
pubmed: 12383085
Mol Plant Microbe Interact. 2003 Aug;16(8):720-6
pubmed: 12906116
Plant J. 2004 Jan;37(1):46-60
pubmed: 14675431
Plant J. 2003 Dec;36(6):867-82
pubmed: 14675451
EMBO J. 2004 Aug 4;23(15):3072-82
pubmed: 15272302
Science. 2006 Jan 13;311(5758):222-6
pubmed: 16373536
Plant Cell. 2006 Feb;18(2):465-76
pubmed: 16377758
Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2851-6
pubmed: 16477026
Nature. 2006 Nov 16;444(7117):323-9
pubmed: 17108957
Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12217-22
pubmed: 17626179
Nature. 2007 Jul 19;448(7151):370-4
pubmed: 17637671
Plant J. 2007 Nov;52(4):595-614
pubmed: 17764515
Biotechniques. 2008 May;44(6):753-6
pubmed: 18476828
J Nematol. 1998 Sep;30(3):309-12
pubmed: 19274224
J Nematol. 1983 Jan;15(1):142-3
pubmed: 19295781
PLoS Pathog. 2009 Aug;5(8):e1000564
pubmed: 19714238
Mol Plant Microbe Interact. 2010 Aug;23(8):991-9
pubmed: 20615110
Mol Cell. 2010 Aug 27;39(4):548-59
pubmed: 20797627
Nat Methods. 2011 Sep 29;8(10):785-6
pubmed: 21959131
Mol Plant. 2012 Sep;5(5):1058-67
pubmed: 22451646
Front Plant Sci. 2011 Nov 22;2:87
pubmed: 22645554
Plant Physiol. 2012 Oct;160(2):944-54
pubmed: 22904163
Nucleic Acids Res. 2013 Jan;41(Database issue):D344-7
pubmed: 23161676
Plant J. 2013 Apr;74(2):185-96
pubmed: 23346875
PLoS Pathog. 2013 Jan;9(1):e1003121
pubmed: 23359647
Plant Signal Behav. 2013 Sep;8(9):null
pubmed: 23803745
Plant Cell. 2013 Sep;25(9):3615-31
pubmed: 24076975
Theor Appl Genet. 1987 Sep;73(5):744-50
pubmed: 24241200
Genome Biol. 2014 Mar 03;15(3):R43
pubmed: 24580726
Annu Rev Phytopathol. 2014;52:243-65
pubmed: 24906126
Front Plant Sci. 2014 Dec 18;5:736
pubmed: 25566304
PLoS One. 2015 Jan 21;10(1):e0115042
pubmed: 25606855
PLoS One. 2015 Apr 07;10(4):e0122256
pubmed: 25849616
Front Plant Sci. 2015 Aug 11;6:623
pubmed: 26322064
New Phytol. 2016 Jul;211(1):138-48
pubmed: 26879496
Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):3389-94
pubmed: 26944079
BMC Microbiol. 2016 Mar 21;16:48
pubmed: 27001199
Genome Biol. 2016 Jun 10;17(1):124
pubmed: 27286965
Plant Physiol. 2017 Feb;173(2):1371-1390
pubmed: 27909045
Microbiology. 2017 Jul;163(7):992-1002
pubmed: 28708051
New Phytol. 2018 Feb;217(3):995-1011
pubmed: 29194634