Nanomechanical DNA resonators for sensing and structural analysis of DNA-ligand complexes.
Antineoplastic Agents
/ chemistry
Cisplatin
/ chemistry
Crystallography, X-Ray
DNA
/ chemistry
Intercalating Agents
/ chemistry
Ligands
Microscopy, Electron, Scanning
Microscopy, Fluorescence
Molecular Dynamics Simulation
Nanomedicine
/ methods
Neoplasms
/ drug therapy
Protein Binding
Stress, Mechanical
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
12 04 2019
12 04 2019
Historique:
received:
23
07
2018
accepted:
18
03
2019
entrez:
14
4
2019
pubmed:
14
4
2019
medline:
10
5
2019
Statut:
epublish
Résumé
The effect of direct or indirect binding of intercalant molecules on DNA structure is of fundamental importance in understanding the biological functioning of DNA. Here we report on self-suspended DNA nanobundles as ultrasensitive nanomechanical resonators for structural studies of DNA-ligand complexes. Such vibrating nanostructures represent the smallest mechanical resonator entirely composed of DNA. A correlative analysis between the mechanical and structural properties is exploited to study the intrinsic changes of double strand DNA, when interacting with different intercalant molecules (YOYO-1 and GelRed) and a chemotherapeutic drug (Cisplatin), at different concentrations. Possible implications of our findings are related to the study of interaction mechanism of a wide category of molecules with DNA, and to further applications in medicine, such as optimal titration of chemotherapeutic drugs and environmental studies for the detection of heavy metals in human serum.
Identifiants
pubmed: 30979901
doi: 10.1038/s41467-019-09612-0
pii: 10.1038/s41467-019-09612-0
pmc: PMC6461617
doi:
Substances chimiques
Antineoplastic Agents
0
Intercalating Agents
0
Ligands
0
DNA
9007-49-2
Cisplatin
Q20Q21Q62J
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1690Références
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