The differential cellular uptake of curcuminoids in vitro depends dominantly on albumin interaction.
Albumins
/ chemistry
Cell Line
Chromatography, Liquid
Curcuma
/ chemistry
Curcumin
/ analogs & derivatives
Diarylheptanoids
Humans
Monocytes
/ drug effects
Nanoparticles
/ chemistry
Polylactic Acid-Polyglycolic Acid Copolymer
/ chemistry
Serum Albumin, Bovine
/ chemistry
Tandem Mass Spectrometry
/ methods
Albumin
Cellular uptakes
Curcuminoids
High-performance liquid chromatography (HPLC)
Protein drug interaction
Journal
Phytomedicine : international journal of phytotherapy and phytopharmacology
ISSN: 1618-095X
Titre abrégé: Phytomedicine
Pays: Germany
ID NLM: 9438794
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
24
10
2018
revised:
21
02
2019
accepted:
21
03
2019
pubmed:
14
4
2019
medline:
17
9
2019
entrez:
14
4
2019
Statut:
ppublish
Résumé
Curcuminoids, mainly present in the plant rhizomes of turmeric (Curcuma longa), consist of mainly three forms (curcumin (CUR), bisdemethoxycurcumin (BDMC) and demethoxycurcumin (DMC)). It has been reported that different forms of curcuminoids possess different biological activities. However, the mechanisms associated with these differences are not well-understood. Recently, our laboratory found differences in the cellular uptake of these curcuminoids. Therefore, it has been inferred that these differences contribute to the different biological activities. In this study, we investigated the mechanisms of differential cellular uptake of these curcuminoids. Based on our previous study, we hypothesized the differential cellular uptake is caused by (I) polarity, (II) transporters, (III) metabolism rate of curcuminoids and (IV) medium components. These four hypotheses were each investigated by (I) neutralizing the polarities of curcuminoids by encapsulation into poly(lactic-co-glycolic) acid nanoparticles (PLGA-NPs), (II) inhibition of polyphenol-related absorption transporters, (III) analysis of the cellular curcuminoids and their metabolites by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and (IV) use of different mediums in cell study. The differential cellular uptake was not affected by (I-III). However, when investigating (IV), not only CUR but also BDMC and DMC were incorporated into cells when serum free media was used. Furthermore, when we used the serum free medium containing bovine serum albumin (BSA), only CUR was taken up but BDMC and DMC were not. Therefore, we identified that the differential cellular uptake of curcuminoids is caused by the medium components, especially BSA. Also, the fluorescence quenching study suggested that differential cellular uptake is due to the different interaction between BSA and each curcuminoid. The differential cellular uptake of curcuminoids was caused by the different interaction between curcuminoids and BSA. The results from this study might give clues on the mechanisms by which curcuminoids exhibit different physiological activities.
Sections du résumé
BACKGROUND
BACKGROUND
Curcuminoids, mainly present in the plant rhizomes of turmeric (Curcuma longa), consist of mainly three forms (curcumin (CUR), bisdemethoxycurcumin (BDMC) and demethoxycurcumin (DMC)). It has been reported that different forms of curcuminoids possess different biological activities. However, the mechanisms associated with these differences are not well-understood. Recently, our laboratory found differences in the cellular uptake of these curcuminoids. Therefore, it has been inferred that these differences contribute to the different biological activities.
PURPOSE
OBJECTIVE
In this study, we investigated the mechanisms of differential cellular uptake of these curcuminoids.
METHOD
METHODS
Based on our previous study, we hypothesized the differential cellular uptake is caused by (I) polarity, (II) transporters, (III) metabolism rate of curcuminoids and (IV) medium components. These four hypotheses were each investigated by (I) neutralizing the polarities of curcuminoids by encapsulation into poly(lactic-co-glycolic) acid nanoparticles (PLGA-NPs), (II) inhibition of polyphenol-related absorption transporters, (III) analysis of the cellular curcuminoids and their metabolites by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and (IV) use of different mediums in cell study.
RESULTS
RESULTS
The differential cellular uptake was not affected by (I-III). However, when investigating (IV), not only CUR but also BDMC and DMC were incorporated into cells when serum free media was used. Furthermore, when we used the serum free medium containing bovine serum albumin (BSA), only CUR was taken up but BDMC and DMC were not. Therefore, we identified that the differential cellular uptake of curcuminoids is caused by the medium components, especially BSA. Also, the fluorescence quenching study suggested that differential cellular uptake is due to the different interaction between BSA and each curcuminoid.
CONCLUSION
CONCLUSIONS
The differential cellular uptake of curcuminoids was caused by the different interaction between curcuminoids and BSA. The results from this study might give clues on the mechanisms by which curcuminoids exhibit different physiological activities.
Identifiants
pubmed: 30981184
pii: S0944-7113(19)30072-8
doi: 10.1016/j.phymed.2019.152902
pii:
doi:
Substances chimiques
Albumins
0
Diarylheptanoids
0
Polylactic Acid-Polyglycolic Acid Copolymer
1SIA8062RS
Serum Albumin, Bovine
27432CM55Q
bisdemethoxycurcumin
2EFO1BP34R
Curcumin
IT942ZTH98
demethoxycurcumin
W2F8059T80
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
152902Informations de copyright
Copyright © 2019. Published by Elsevier GmbH.