Programming of ES cells and reprogramming of fibroblasts into renal lineage-like cells.
Differentiation
Direct conversion
Intermediate mesoderm-like cells
Renal epithelial-like cells
Journal
Experimental cell research
ISSN: 1090-2422
Titre abrégé: Exp Cell Res
Pays: United States
ID NLM: 0373226
Informations de publication
Date de publication:
15 06 2019
15 06 2019
Historique:
received:
02
12
2018
revised:
06
04
2019
accepted:
08
04
2019
pubmed:
15
4
2019
medline:
2
6
2020
entrez:
15
4
2019
Statut:
ppublish
Résumé
This study aims to prepare intermediate mesoderm-like cells from mouse embryonic fibroblasts (MEFs). In the first step, intermediate mesoderm-like cells (IMLCs) and renal epithelial-like cells (RELCs) were extracted from mouse embryonic stem cells (mESCs) in a specified media that contained two small molecules, CHIR99021 and TTNPB, along with growth factors, FGF9and BMP7. Then, MEFs were directly converted into IM by genes for the pluripotency factors, which encode the transcription factors; Oct4, Sox2, Klf4, and c-Myc (OSKM). These unstable intermediate cells were quickly encouraged to form IM with the assistance of CHIR99021 and TTNPB. The results showed that exogenous expression of OSKM factors for four days was adequate to generate partially reprogrammed cells (SSEA1
Identifiants
pubmed: 30981668
pii: S0014-4827(19)30185-5
doi: 10.1016/j.yexcr.2019.04.011
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
225-234Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.