Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection.
Adult
Female
Haplotypes
/ genetics
Hepatitis B virus
/ isolation & purification
Hepatitis B, Chronic
/ virology
Hepatitis D, Chronic
/ virology
Hepatitis Delta Virus
/ isolation & purification
Humans
Male
Quasispecies
/ genetics
Superinfection
/ virology
Trans-Activators
/ genetics
Viral Regulatory and Accessory Proteins
Virus Replication
/ genetics
Hepatitis B X gene
Hepatitis B virus
Hepatitis B virus-hepatitis delta virus interaction
Hepatitis delta virus
Next-generation sequencing
Viral quasispecies
Journal
World journal of gastroenterology
ISSN: 2219-2840
Titre abrégé: World J Gastroenterol
Pays: United States
ID NLM: 100883448
Informations de publication
Date de publication:
07 Apr 2019
07 Apr 2019
Historique:
received:
24
12
2018
revised:
25
02
2019
accepted:
01
03
2019
entrez:
16
4
2019
pubmed:
16
4
2019
medline:
25
6
2019
Statut:
ppublish
Résumé
Hepatitis delta virus (HDV) seems to strongly suppress hepatitis B virus (HBV) replication, although little is known about the mechanism of this interaction. Both these viruses show a dynamic distribution of mutants, resulting in viral quasispecies. Next-generation sequencing is a viable approach for analyzing the composition of these mutant spectra. As the regulatory hepatitis B X protein (HBx) is essential for HBV replication, determination of HBV X gene ( To compare HBV quasispecies complexity in the Twenty-four untreated patients were included: 7/24 (29.2%) with HBeAg-negative chronic HBV infection (CI, previously termed inactive carriers), 8/24 (33.3%) with HBeAg-negative chronic hepatitis B (CHB) and 9/24 (37.5%) with CHD. A serum sample from each patient was first tested for HBV DNA levels. The CHB patients showed higher median HBV-DNA levels [5.4 logIU/mL, interquartile range (IQR) 3.5-7.9] than CHD (3.4 logIU/mL, IQR 3-7.6) ( The lower-replication CHD and CI groups show a trend to higher quasispecies complexity than the higher-replication CHB group. The mechanisms associated with this greater complexity require elucidation.
Sections du résumé
BACKGROUND
BACKGROUND
Hepatitis delta virus (HDV) seems to strongly suppress hepatitis B virus (HBV) replication, although little is known about the mechanism of this interaction. Both these viruses show a dynamic distribution of mutants, resulting in viral quasispecies. Next-generation sequencing is a viable approach for analyzing the composition of these mutant spectra. As the regulatory hepatitis B X protein (HBx) is essential for HBV replication, determination of HBV X gene (
AIM
OBJECTIVE
To compare HBV quasispecies complexity in the
METHODS
METHODS
Twenty-four untreated patients were included: 7/24 (29.2%) with HBeAg-negative chronic HBV infection (CI, previously termed inactive carriers), 8/24 (33.3%) with HBeAg-negative chronic hepatitis B (CHB) and 9/24 (37.5%) with CHD. A serum sample from each patient was first tested for HBV DNA levels. The
RESULTS
RESULTS
CHB patients showed higher median HBV-DNA levels [5.4 logIU/mL, interquartile range (IQR) 3.5-7.9] than CHD (3.4 logIU/mL, IQR 3-7.6) (
CONCLUSION
CONCLUSIONS
The lower-replication CHD and CI groups show a trend to higher quasispecies complexity than the higher-replication CHB group. The mechanisms associated with this greater complexity require elucidation.
Identifiants
pubmed: 30983817
doi: 10.3748/wjg.v25.i13.1566
pmc: PMC6452231
doi:
Substances chimiques
Trans-Activators
0
Viral Regulatory and Accessory Proteins
0
hepatitis B virus X protein
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1566-1579Déclaration de conflit d'intérêts
Conflict-of-interest statement: Josep Gregori is an employee of Roche Diagnostics, SL.
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