MicroED data collection with SerialEM.
Electron diffraction
MicroED
Microcrystal
SerialEM
Transmission electron microscopy
cryoEM
Journal
Ultramicroscopy
ISSN: 1879-2723
Titre abrégé: Ultramicroscopy
Pays: Netherlands
ID NLM: 7513702
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
08
01
2019
revised:
04
03
2019
accepted:
18
03
2019
pubmed:
16
4
2019
medline:
21
12
2019
entrez:
16
4
2019
Statut:
ppublish
Résumé
The cryoEM method Microcrystal Electron Diffraction (MicroED) involves transmission electron microscope (TEM) and electron detector working in synchrony to collect electron diffraction data by continuous rotation. We previously reported several protein, peptide, and small molecule structures by MicroED using manual control of the microscope and detector to collect data. Here we present a procedure to automate this process using a script developed for the popular open-source software package SerialEM. With this approach, SerialEM coordinates stage rotation, microscope operation, and camera functions for automated continuous-rotation MicroED data collection. Depending on crystal and substrate geometry, more than 300 datasets can be collected overnight in this way, facilitating high-throughput MicroED data collection for large-scale data analyses.
Identifiants
pubmed: 30986656
pii: S0304-3991(19)30002-6
doi: 10.1016/j.ultramic.2019.03.009
pmc: PMC6752703
mid: NIHMS1526950
pii:
doi:
Substances chimiques
Peptides
0
Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
77-80Subventions
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.
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