Toxicological assessment of magnesium oxide nanoparticles in HT29 intestinal cells.
Flow cytometry
HT29 cells
Magnesium oxide
Nanoparticles
Nanotoxicology
Oral exposure
Journal
Archives of toxicology
ISSN: 1432-0738
Titre abrégé: Arch Toxicol
Pays: Germany
ID NLM: 0417615
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
15
11
2018
accepted:
09
04
2019
pubmed:
17
4
2019
medline:
22
7
2020
entrez:
17
4
2019
Statut:
ppublish
Résumé
Nanoparticles (NPs) are increasingly used in different consumer-related areas, for instance in food packaging or as additives, because of their enormous potential. Magnesium oxide (MgO) is an EU-approved food additive (E number 530). It is commonly used as a drying agent for powdered foods, for colour retention or as a food supplement. There are no consistent results regarding the effects of oral MgO NP uptake. Consequently, the aim of this study was to examine the effects of MgO NPs in the HT29 intestinal cell line. MgO NP concentrations ranged from 0.001 to 100 μg/ml and incubation times were up to 24 h. The cytotoxic and genotoxic potential were investigated. Apoptotic processes and cell cycle changes were analysed by flow cytometry. Finally, oxidative stress was examined. Transmission electron microscopy indicated that there was no cellular uptake. MgO NPs had no cytotoxic or genotoxic effects in HT29 cells and they did not induce apoptotic processes, cell cycle changes or oxidative stress.
Identifiants
pubmed: 30989313
doi: 10.1007/s00204-019-02451-4
pii: 10.1007/s00204-019-02451-4
doi:
Substances chimiques
Mutagens
0
Magnesium Oxide
3A3U0GI71G
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1491-1500Références
Alqahtani S, Alomar SY (2017) Induction of apoptosis and cytokine markers in colon cancer cells by magnesium oxide (MgO) nanoparticles. Toxicol Eviron Chem 99(2):302–314
doi: 10.1080/02772248.2016.1169684
AshaRani PV, Low Kah Mun G, Hande MP, Valiyaveettil S (2009) Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano 3(2):279–290. https://doi.org/10.1021/nn800596w
doi: 10.1021/nn800596w
pubmed: 19236062
Auffan M, Rose J, Bottero JY, Lowry GV, Jolivet JP, Wiesner MR (2009) Towards a definition of inorganic nanoparticles from an environmental, health and safety perspective. Nat Nanotechnol 4(10):634–641. https://doi.org/10.1038/nnano.2009.242
doi: 10.1038/nnano.2009.242
pubmed: 19809453
Beckman Coulter Inc (2017) Annexin A5 FITC/7-AAD KIT. B60224–AA
Bertinato J, Plouffe LJ, Lavergne C, Ly C (2014) Bioavailability of magnesium from inorganic and organic compounds is similar in rats fed a high phytic acid diet. Magn Res 27(4):175–185. https://doi.org/10.1684/mrh.2014.0374
doi: 10.1684/mrh.2014.0374
Cao Y, Li J, Liu F et al (2016) Consideration of interaction between nanoparticles and food components for the safety assessment of nanoparticles following oral exposure: a review. Environ Toxicol Pharmacol 46:206–210. https://doi.org/10.1016/j.etap.2016.07.023
doi: 10.1016/j.etap.2016.07.023
pubmed: 27497726
Chaudhry Q, Scotter M, Blackburn J et al (2008) Applications and implications of nanotechnologies for the food sector. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 25(3):241–258. https://doi.org/10.1080/02652030701744538
doi: 10.1080/02652030701744538
pubmed: 18311618
Chen M, von Mikecz A (2005) Formation of nucleoplasmic protein aggregates impairs nuclear function in response to SiO
doi: 10.1016/j.yexcr.2004.12.021
pubmed: 15777787
Cushen M, Kerry J, Morris M, Cruz-Romero M, Cummins E (2012) Nanotechnologies in the food industry—recent developments, risks and regulation. Trends Food Sci Tech 24(1):30–46. https://doi.org/10.1016/j.tifs.2011.10.006
doi: 10.1016/j.tifs.2011.10.006
Date AA, Hanes J, Ensign LM (2016) Nanoparticles for oral delivery: design, evaluation and state-of-the-art. J Control Release 240:504–526. https://doi.org/10.1016/j.jconrel.2016.06.016
doi: 10.1016/j.jconrel.2016.06.016
pubmed: 27292178
pmcid: 5064878
De Matteis V (2017) Exposure to inorganic nanoparticles: routes of entry, immune response, biodistribution and in vitro/in vivo toxicity evaluation. Toxics. https://doi.org/10.3390/toxics5040029
doi: 10.3390/toxics5040029
pubmed: 29051461
pmcid: 5750557
DeLoid GM, Cohen JM, Pyrgiotakis G, Demokritou P (2017) Preparation, characterization, and in vitro dosimetry of dispersed, engineered nanomaterials. Nat Protoc 12(2):355–371. https://doi.org/10.1038/nprot.2016.172
doi: 10.1038/nprot.2016.172
pubmed: 28102836
pmcid: 5857388
Elsaesser A, Howard CV (2012) Toxicology of nanoparticles. Adv Drug Deliv Rev 64(2):129–137. https://doi.org/10.1016/j.addr.2011.09.001
doi: 10.1016/j.addr.2011.09.001
pubmed: 21925220
Emerich DF (2005) Nanomedicine–prospective therapeutic and diagnostic applications. Expert Opin Biol Ther 5(1):1–5. https://doi.org/10.1517/14712598.5.1.1
doi: 10.1517/14712598.5.1.1
pubmed: 15709905
Federal Ministry of Justice and Consumer Protection (2012) Verordnung über die Zulassung von Zusatzstoffen zu Lebensmitteln zu technologischen Zwecken (Zusatzstoff- Zulassungsverordnung - ZZulV), pp 1–92
Ge S, Wang G, Shen Y et al (2011) Cytotoxic effects of MgO nanoparticles on human umbilical vein endothelial cells in vitro. IET Nanobiotechnol 5(2):36. https://doi.org/10.1049/iet-nbt.2010.0022
doi: 10.1049/iet-nbt.2010.0022
pubmed: 21495778
Gerloff K, Albrecht C, Boots AW, Forster I, Schins RPF (2009) Cytotoxicity and oxidative DNA damage by nanoparticles in human intestinal Caco-2 cells. Nanotoxicology 3(4):355–364. https://doi.org/10.3109/17435390903276933
doi: 10.3109/17435390903276933
Ghobadian M, Nabiuni M, Parivar K, Fathi M, Pazooki J (2015) Toxic effects of magnesium oxide nanoparticles on early developmental and larval stages of zebrafish (Danio rerio). Ecotoxicol Environ Saf 122:260–267. https://doi.org/10.1016/j.ecoenv.2015.08.009
doi: 10.1016/j.ecoenv.2015.08.009
pubmed: 26283286
Glei M, Schneider T, Schlormann W (2016) Comet assay: an essential tool in toxicological research. Arch Toxicol 90(10):2315–2336. https://doi.org/10.1007/s00204-016-1767-y
doi: 10.1007/s00204-016-1767-y
pubmed: 27378090
Hobson DW (2009) Commercialization of nanotechnology. Wiley Interdiscip Rev Nanomed Nanobiotechnol 1(2):189–202. https://doi.org/10.1002/wnan.28
doi: 10.1002/wnan.28
pubmed: 20049790
Hoshyar N, Gray S, Han H, Bao G (2016) The effect of nanoparticle size on in vivo pharmacokinetics and cellular interaction. Nanomed (Lond) 11(6):673–692. https://doi.org/10.2217/nnm.16.5
doi: 10.2217/nnm.16.5
Huang YW, Cambre M, Lee HJ (2017) The toxicity of nanoparticles depends on multiple molecular and physicochemical mechanisms. Int J Mol Sci. https://doi.org/10.3390/ijms18122702
doi: 10.3390/ijms18122702
pubmed: 29301217
pmcid: 5796057
Kalyanaraman B, Darley-Usmar V, Davies KJ et al (2012) Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations. Free Radic Biol Med 52(1):1–6. https://doi.org/10.1016/j.freeradbiomed.2011.09.030
doi: 10.1016/j.freeradbiomed.2011.09.030
pubmed: 22027063
Kang SJ, Kim BM, Lee YJ, Chung HW (2008) Titanium dioxide nanoparticles trigger p53-mediated damage response in peripheral blood lymphocytes. Environ Mol Mutagen 49(5):399–405. https://doi.org/10.1002/em.20399
doi: 10.1002/em.20399
pubmed: 18418868
Krishnamoorthy K, Moon JY, Hyun HB, Cho SK, Kim SJ (2012) Mechanistic investigation on the toxicity of MgO nanoparticles toward cancer cells. J Mater Chem 22(47):24610–24617. https://doi.org/10.1039/c2jm35087d
doi: 10.1039/c2jm35087d
Kumaran RS, Choi YK, Singh V et al (2015) In Vitro cytotoxic evaluation of MgO nanoparticles and their effect on the expression of ROS genes. Int J Mol Sci 16(4):7551–7564. https://doi.org/10.3390/ijms16047551
doi: 10.3390/ijms16047551
pubmed: 25854426
pmcid: 4425033
Lai JC, Lai MB, Jandhyam S et al (2008) Exposure to titanium dioxide and other metallic oxide nanoparticles induces cytotoxicity on human neural cells and fibroblasts. Int J Nanomed 3(4):533–545
Mahmoud A, Ezgi O, Merve A, Ozhan G (2016) In vitro toxicological assessment of magnesium oxide nanoparticle exposure in several Mammalian cell types. Int J Toxicol 35(4):429–437. https://doi.org/10.1177/1091581816648624
doi: 10.1177/1091581816648624
pubmed: 27177543
Mahmoudi M, Azadmanesh K, Shokrgozar MA, Journeay WS, Laurent S (2011) Effect of nanoparticles on the cell life cycle. Chem Rev 111(5):3407–3432. https://doi.org/10.1021/cr1003166
doi: 10.1021/cr1003166
pubmed: 21401073
Mangalampalli B, Dumala N, Perumalla Venkata R, Grover P (2018) Genotoxicity, biochemical, and biodistribution studies of magnesium oxide nano and microparticles in albino wistar rats after 28-day repeated oral exposure. Environ Toxicol 33(4):396–410. https://doi.org/10.1002/tox.22526
doi: 10.1002/tox.22526
pubmed: 29282847
McClements DJ, Xiao H (2017) Is nano safe in foods? Establishing the factors impacting the gastrointestinal fate and toxicity of organic and inorganic food-grade nanoparticles. NPJ Sci Food 1(1):6. https://doi.org/10.1038/s41538-017-0005-1
doi: 10.1038/s41538-017-0005-1
pubmed: 31304248
pmcid: 6548419
Moeini-Nodeh S, Rahimifard M, Baeeri M, Abdollahi M (2016) Functional improvement in rats’ pancreatic islets using magnesium oxide nanoparticles through antiapoptotic and antioxidant pathways. Biol Trace Elem Res. https://doi.org/10.1007/s12011-016-0754-8
doi: 10.1007/s12011-016-0754-8
pubmed: 27234250
Moghimi SM, Hunter AC, Murray JC (2005) Nanomedicine: current status and future prospects. FASEB J 19(3):311–330. https://doi.org/10.1096/fj.04-2747rev
doi: 10.1096/fj.04-2747rev
pubmed: 15746175
Morgan K (2005) Development of a preliminary framework for informing the risk analysis and risk management of nanoparticles. Risk Anal 25(6):1621–1635. https://doi.org/10.1111/j.1539-6924.2005.00681.x
doi: 10.1111/j.1539-6924.2005.00681.x
pubmed: 16506988
Neuhaus B, Tosun B, Rotan O, Frede A, Westendorf AM, Epple M (2016) Nanoparticles as transfection agents: a comprehensive study with ten different cell lines. RSC Adv 6(22):18102–18112. https://doi.org/10.1039/c5ra25333k
doi: 10.1039/c5ra25333k
Pozarowski P, Darzynkiewicz Z (2004) Analysis of cell cycle by flow cytometry. Methods Mol Biol 281:301–311. https://doi.org/10.1385/1-59259-811-0:301
doi: 10.1385/1-59259-811-0:301
pubmed: 15220539
Quamme GA (2008) Recent developments in intestinal magnesium absorption. Curr Opin Gastroenterol 24(2):230–235. https://doi.org/10.1097/MOG.0b013e3282f37b59
doi: 10.1097/MOG.0b013e3282f37b59
pubmed: 18301276
Schieber M, Chandel NS (2014) ROS function in redox signaling and oxidative stress. Curr Biol 24(10):R453–R462. https://doi.org/10.1016/j.cub.2014.03.034
doi: 10.1016/j.cub.2014.03.034
pubmed: 24845678
pmcid: 4055301
Schneider T, Westermann M, Glei M (2017) In vitro uptake and toxicity studies of metal nanoparticles and metal oxide nanoparticles in human HT29 cells. Arch Toxicol. https://doi.org/10.1007/s00204-017-1976-z
doi: 10.1007/s00204-017-1976-z
pubmed: 28924833
pmcid: 5773661
Scientific Committee on Emerging and Newly Identified Health Risks (2010) Scientific basis for the definition of the term “nanomaterial”. European Commission, Luxembourg
Shao XR, Wei XQ, Song X et al (2015) Independent effect of polymeric nanoparticle zeta potential/surface charge, on their cytotoxicity and affinity to cells. Cell Prolif 48(4):465–474. https://doi.org/10.1111/cpr.12192
doi: 10.1111/cpr.12192
pubmed: 26017818
Singh N, Manshian B, Jenkins GJ et al (2009) NanoGenotoxicology: the DNA damaging potential of engineered nanomaterials. Biomaterials 30(23–24):3891–3914. https://doi.org/10.1016/j.biomaterials.2009.04.009
doi: 10.1016/j.biomaterials.2009.04.009
pubmed: 19427031
Smolkova B, El Yamani N, Collins AR, Gutleb AC, Dusinska M (2015) Nanoparticles in food. Epigenetic changes induced by nanomaterials and possible impact on health. Food Chem Toxicol 77:64–73. https://doi.org/10.1016/j.fct.2014.12.015
doi: 10.1016/j.fct.2014.12.015
pubmed: 25554528
Sonkaria S, Ahn SH, Khare V (2012) Nanotechnology and its impact on food and nutrition: a review. Recent Pat Food Nutr Agric 4(1):8–18
doi: 10.2174/1876142911204010008
Sun J, Wang S, Zhao D, Hun FH, Weng L, Liu H (2011) Cytotoxicity, permeability, and inflammation of metal oxide nanoparticles in human cardiac microvascular endothelial cells: cytotoxicity, permeability, and inflammation of metal oxide nanoparticles. Cell Biol Toxicol 27(5):333–342. https://doi.org/10.1007/s10565-011-9191-9
doi: 10.1007/s10565-011-9191-9
pubmed: 21681618
The European Commission (2011) Comission recommendation of 18 October 2011 on the definition of nanomaterial. Off J Eur Union L275:38–40
The Nanodatabase (2018) http://nanodb.dk/en/search-database/ . Accessed 10 Oct 2018
Uysal N, Kizildag S, Yuce Z et al (2018) Timeline (bioavailability) of magnesium compounds in hours: which magnesium compound works best? Biol Trace Elem Res. https://doi.org/10.1007/s12011-018-1351-9
doi: 10.1007/s12011-018-1351-9
pubmed: 29679349
van der Merwe D, Tawde S, Pickrell JA, Erickson LE (2009) Nanocrystalline titanium dioxide and magnesium oxide in vitro dermal absorption in human skin. Cutan Ocul Toxicol 28(2):78–82. https://doi.org/10.1080/15569520902914926
doi: 10.1080/15569520902914926
pubmed: 19514931
Wetteland CL, Nguyen NY, Liu H (2016) Concentration-dependent behaviors of bone marrow derived mesenchymal stem cells and infectious bacteria toward magnesium oxide nanoparticles. Acta Biomater 35:341–356. https://doi.org/10.1016/j.actbio.2016.02.032
doi: 10.1016/j.actbio.2016.02.032
pubmed: 26923529
Wilhelmi V, Fischer U, van Berlo D, Schulze-Osthoff K, Schins RP, Albrecht C (2012) Evaluation of apoptosis induced by nanoparticles and fine particles in RAW 264.7 macrophages: facts and artefacts. Toxicol In Vitro 26(2):323–334. https://doi.org/10.1016/j.tiv.2011.12.006
doi: 10.1016/j.tiv.2011.12.006
pubmed: 22198050
Willers J, Heinemann M, Bitterlich N, Hahn A (2015) Intake of minerals from food supplements in a German population—a nationwide survey. Food Nutr Sci 6:205–215
Wishart DS, Feunang YD, Guo AC et al (2018) DrugBank 5.0: a major update to the DrugBank database for 2018. Nucleic Acids Res 46(1):1074–1082. https://doi.org/10.1093/nar/gkx1037
doi: 10.1093/nar/gkx1037
Yamasaki M, Funakoshi S, Matsuda S et al (2014) Interaction of magnesium oxide with gastric acid secretion inhibitors in clinical pharmacotherapy. Eur J Clin Pharmacol 70(8):921–924. https://doi.org/10.1007/s00228-014-1694-x
doi: 10.1007/s00228-014-1694-x
pubmed: 24820768
Zhang J, Tang H, Liu Z, Chen B (2017) Effects of major parameters of nanoparticles on their physical and chemical properties and recent application of nanodrug delivery system in targeted chemotherapy. Int J Nanomed 12:8483–8493. https://doi.org/10.2147/IJN.S148359
doi: 10.2147/IJN.S148359