Activation profiles of monocyte-macrophages and HDL function in healthy women in relation to menstrual cycle and in polycystic ovary syndrome patients.
Cholesterol efflux
Macrophage activation
Macrophages
Menstrual cycle
Monocytes
Polycystic ovary syndrome
Journal
Endocrine
ISSN: 1559-0100
Titre abrégé: Endocrine
Pays: United States
ID NLM: 9434444
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
05
12
2018
accepted:
19
03
2019
pubmed:
18
4
2019
medline:
28
5
2020
entrez:
18
4
2019
Statut:
ppublish
Résumé
Hormonal status and menopause affect human macrophage function and cardiometabolic risk. In polycystic ovary syndrome (PCOS) patients the cardiometabolic risk increases through mechanisms that are largely unknown. We tested the hypotheses that macrophage activation is influenced by menstrual cycle and that ovarian dysfunction in PCOS patients is associated with altered macrophage inflammatory responses and cholesterol efflux capacity of serum HDL. Blood samples were obtained in the follicular and luteal phases from cycling women (n = 10) and on a single visit from PCOS patients with ovarian dysfunction (n = 11). Monocyte-derived macrophage activation and monocyte subsets were characterized ex vivo using flow cytometry. The capacity of HDL to promote cell cholesterol efflux through the main efflux pathways, namely aqueous diffusion, ATP-binding cassette A1 and G1, was also evaluated. Hormone and metabolic profiles differed as expected in relation to menstrual cycle and ovulatory dysfunction. Overall, macrophage responses to activating stimuli in PCOS patients were blunted compared with cycling women. Macrophages in the follicular phase were endowed with enhanced responsiveness to LPS/interferon-γ compared with the luteal phase and PCOS. These changes were not related to baseline differences in monocytes. HDL cholesterol efflux capacity through multiple pathways was significantly impaired in PCOS patients compared to healthy women, at least in part independent from lower HDL-cholesterol levels. Regular menstrual cycles entailed fluctuations in macrophage activation. Such dynamic pattern was attenuated in PCOS. Along with impaired HDL function, this may contribute to the increased cardiometabolic risk associated with PCOS.
Identifiants
pubmed: 30993600
doi: 10.1007/s12020-019-01911-2
pii: 10.1007/s12020-019-01911-2
doi:
Substances chimiques
Lipoproteins, HDL
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
360-369Subventions
Organisme : Università degli Studi di Padova
ID : MIUR
Pays : International
Organisme : Università degli Studi di Parma
ID : MIUR
Pays : International
Organisme : Università degli Studi di Padova
ID : MIUR
Pays : International
Références
Circulation. 2017 Jun 20;135(25):2494-2504
pubmed: 28450350
Curr Opin Pharmacol. 2013 Aug;13(4):576-81
pubmed: 23731522
Horm Res Paediatr. 2017;88(6):371-395
pubmed: 29156452
N Engl J Med. 2011 Jan 13;364(2):127-35
pubmed: 21226578
N Engl J Med. 2016 Jul 7;375(1):54-64
pubmed: 27406348
Mediators Inflamm. 2018 Feb 8;2018:2868702
pubmed: 29576743
Eur J Obstet Gynecol Reprod Biol. 1991 May 10;39(3):203-7
pubmed: 1709605
Clin Endocrinol (Oxf). 2017 May;86(5):739-746
pubmed: 28199736
Arthritis Rheumatol. 2015 May;67(5):1155-64
pubmed: 25605003
J Clin Endocrinol Metab. 2015 Jan;100(1):E50-8
pubmed: 25303489
J Clin Endocrinol Metab. 2010 May;95(5):2038-49
pubmed: 20375205
Br Med J. 1975 Jul 12;3(5975):102
pubmed: 1139226
Hum Reprod. 2014 Jul;29(7):1518-25
pubmed: 24842896
Ther Clin Risk Manag. 2017 Dec 11;13:1555-1562
pubmed: 29270015
J Clin Invest. 2004 Nov;114(9):1343-53
pubmed: 15520867
J Steroid Biochem Mol Biol. 2006 Jul;100(1-3):59-66
pubmed: 16730436
Ann Rheum Dis. 2014 Mar;73(3):609-15
pubmed: 23562986
Curr Pharm Biotechnol. 2012 Feb;13(2):292-302
pubmed: 21470124
Horm Metab Res. 2013 Jun;45(6):423-9
pubmed: 23430591
Circ Res. 2016 Feb 19;118(4):653-67
pubmed: 26892964
Biol Reprod. 2018 Oct 1;99(4):838-852
pubmed: 29688269
Am J Physiol Heart Circ Physiol. 2018 Dec 1;315(6):H1569-H1588
pubmed: 30216121
Lipids Health Dis. 2011 Oct 28;10:193
pubmed: 22035022
Circulation. 1999 Dec 7;100(23):2319-25
pubmed: 10587335
J Clin Endocrinol Metab. 2013 Dec;98(12):4565-92
pubmed: 24151290
Front Pharmacol. 2018 Feb 22;9:71
pubmed: 29520230
Autoimmunity. 2015 May;48(3):139-51
pubmed: 25811915
J Clin Endocrinol Metab. 2014 May;99(5):E841-7
pubmed: 24512495
J Am Coll Cardiol. 2016 May 31;67(21):2480-7
pubmed: 27230043
J Leukoc Biol. 2018 Mar 30;:null
pubmed: 29603382
Circulation. 2018 Mar 20;137(12):e67-e492
pubmed: 29386200
Atherosclerosis. 2017 Nov;266:95-102
pubmed: 29017104
Diabetologia. 2013 Aug;56(8):1856-66
pubmed: 23616239
Fertil Steril. 2004 Mar;81 Suppl 1:876-81
pubmed: 15019823
J Clin Endocrinol Metab. 2007 Feb;92(2):405-13
pubmed: 17090633
Mol Cell Endocrinol. 2013 Aug 15;375(1-2):121-9
pubmed: 23707618
Fertil Steril. 2014 May;101(5):1383-91
pubmed: 24581581
Arterioscler Thromb Vasc Biol. 2013 Jun;33(6):1127-34
pubmed: 23640494
Am J Obstet Gynecol. 2000 Oct;183(4):967-73
pubmed: 11035348
Circ Cardiovasc Genet. 2012 Feb 1;5(1):42-50
pubmed: 22062970
Circ Res. 2018 Jan 5;122(1):113-127
pubmed: 29301844