Activation profiles of monocyte-macrophages and HDL function in healthy women in relation to menstrual cycle and in polycystic ovary syndrome patients.


Journal

Endocrine
ISSN: 1559-0100
Titre abrégé: Endocrine
Pays: United States
ID NLM: 9434444

Informations de publication

Date de publication:
11 2019
Historique:
received: 05 12 2018
accepted: 19 03 2019
pubmed: 18 4 2019
medline: 28 5 2020
entrez: 18 4 2019
Statut: ppublish

Résumé

Hormonal status and menopause affect human macrophage function and cardiometabolic risk. In polycystic ovary syndrome (PCOS) patients the cardiometabolic risk increases through mechanisms that are largely unknown. We tested the hypotheses that macrophage activation is influenced by menstrual cycle and that ovarian dysfunction in PCOS patients is associated with altered macrophage inflammatory responses and cholesterol efflux capacity of serum HDL. Blood samples were obtained in the follicular and luteal phases from cycling women (n = 10) and on a single visit from PCOS patients with ovarian dysfunction (n = 11). Monocyte-derived macrophage activation and monocyte subsets were characterized ex vivo using flow cytometry. The capacity of HDL to promote cell cholesterol efflux through the main efflux pathways, namely aqueous diffusion, ATP-binding cassette A1 and G1, was also evaluated. Hormone and metabolic profiles differed as expected in relation to menstrual cycle and ovulatory dysfunction. Overall, macrophage responses to activating stimuli in PCOS patients were blunted compared with cycling women. Macrophages in the follicular phase were endowed with enhanced responsiveness to LPS/interferon-γ compared with the luteal phase and PCOS. These changes were not related to baseline differences in monocytes. HDL cholesterol efflux capacity through multiple pathways was significantly impaired in PCOS patients compared to healthy women, at least in part independent from lower HDL-cholesterol levels. Regular menstrual cycles entailed fluctuations in macrophage activation. Such dynamic pattern was attenuated in PCOS. Along with impaired HDL function, this may contribute to the increased cardiometabolic risk associated with PCOS.

Identifiants

pubmed: 30993600
doi: 10.1007/s12020-019-01911-2
pii: 10.1007/s12020-019-01911-2
doi:

Substances chimiques

Lipoproteins, HDL 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

360-369

Subventions

Organisme : Università degli Studi di Padova
ID : MIUR
Pays : International
Organisme : Università degli Studi di Parma
ID : MIUR
Pays : International
Organisme : Università degli Studi di Padova
ID : MIUR
Pays : International

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Auteurs

Serena Tedesco (S)

Venetian Institute of Molecular Medicine, Padova, Italy.

Maria Pia Adorni (MP)

Department of Food and Drug, University of Parma, Parma, Italy.

Nicoletta Ronda (N)

Department of Food and Drug, University of Parma, Parma, Italy.

Roberta Cappellari (R)

Venetian Institute of Molecular Medicine, Padova, Italy.

Roberto Mioni (R)

Clinica Medica 3, University Hospital, Padova, Italy.

Mattia Barbot (M)

Endocrinology Unit, University Hospital, Padova, Italy.

Silvia Pinelli (S)

Endocrinology Unit, University Hospital, Padova, Italy.

Mario Plebani (M)

Department of Medicine, University of Padova, Padova, Italy.

Chiara Bolego (C)

Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.

Carla Scaroni (C)

Endocrinology Unit, University Hospital, Padova, Italy.
Department of Medicine, University of Padova, Padova, Italy.

Franco Bernini (F)

Department of Food and Drug, University of Parma, Parma, Italy.

Gian Paolo Fadini (GP)

Venetian Institute of Molecular Medicine, Padova, Italy.
Department of Medicine, University of Padova, Padova, Italy.

Andrea Cignarella (A)

Department of Medicine, University of Padova, Padova, Italy. andrea.cignarella@unipd.it.

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Classifications MeSH