Combination of Double-Mediator System with Large-Scale Integration-Based Amperometric Devices for Detecting NAD(P)H:quinone Oxidoreductase 1 Activity of Cancer Cell Aggregates.
Cell Respiration
/ physiology
Electrochemical Techniques
/ instrumentation
Electron Transport Complex I
/ antagonists & inhibitors
Enzyme Assays
/ instrumentation
Ferricyanides
/ chemistry
Humans
MCF-7 Cells
NAD(P)H Dehydrogenase (Quinone)
/ analysis
Optical Imaging
/ instrumentation
Oxidation-Reduction
Rotenone
/ pharmacology
Vitamin K
/ analogs & derivatives
Vitamin K 3
/ chemistry
NAD(P)H:quinone oxidoreductase 1
cell aggregates
cell analysis
double-mediator system
large-scale integration-based amperometric device
Journal
ACS sensors
ISSN: 2379-3694
Titre abrégé: ACS Sens
Pays: United States
ID NLM: 101669031
Informations de publication
Date de publication:
28 06 2019
28 06 2019
Historique:
pubmed:
18
4
2019
medline:
25
4
2020
entrez:
18
4
2019
Statut:
ppublish
Résumé
NAD(P)H:quinone oxidoreductase 1 (NQO1) is a key enzyme providing cytoprotection from quinone species. In addition, it is expressed at high levels in many human tumors, such as breast cancer. Therefore, it is considered to be a potential target in cancer treatment. In order to detect intracellular NQO1 activity in MCF-7 aggregates as a cancer model, we present, in this study, a double-mediator system combined with large-scale integration (LSI)-based amperometric devices. This LSI device contained 20 × 20 Pt working electrodes with a 250 μm pitch for electrochemical imaging. In the detection system, menadione (MD) and [Fe(CN)
Identifiants
pubmed: 30995391
doi: 10.1021/acssensors.9b00344
doi:
Substances chimiques
Ferricyanides
0
Rotenone
03L9OT429T
Vitamin K
12001-79-5
hexacyanoferrate III
13408-62-3
Vitamin K 3
723JX6CXY5
NAD(P)H Dehydrogenase (Quinone)
EC 1.6.5.2
NQO1 protein, human
EC 1.6.5.2
Electron Transport Complex I
EC 7.1.1.2
menadiol
VQ093653DO
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM