Induction of EGFP expression in Pichia pastoris during co-culture with human endothelial cell line.
Co-culture
Endothelial cell
Green fluorescent protein
NF-κB
Pichia pastoris
Journal
Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
04
01
2019
revised:
19
03
2019
accepted:
07
04
2019
pubmed:
18
4
2019
medline:
2
6
2020
entrez:
18
4
2019
Statut:
ppublish
Résumé
While Pichia pastoris has been developed into a versatile recombinant protein expression system, there are only few studies that have investigated the efficacious use of this yeast with human cells. In this study, we demonstrated that P. pastoris can be cultured under mammalian cell culture conditions and co-cultured with human endothelial cells. Co-cultures did not affect endothelial cell morphology or viability. Additionally, P. pastoris was induced to express enhanced green fluorescence protein when co-cultured with human endothelial cell line EA.hy926 under mammalian cell culture conditions. Our study provides data to support the use of P. pastoris as a vehicle for direct delivery of recombinant proteins to mammalian cells during co-culture.
Identifiants
pubmed: 30995456
pii: S0167-7012(19)30009-0
doi: 10.1016/j.mimet.2019.04.006
pii:
doi:
Substances chimiques
Recombinant Proteins
0
enhanced green fluorescent protein
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
28-34Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.