A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies.
difference X-ray scattering
protein structural changes
Journal
Journal of applied crystallography
ISSN: 0021-8898
Titre abrégé: J Appl Crystallogr
Pays: United States
ID NLM: 9876190
Informations de publication
Date de publication:
01 Apr 2019
01 Apr 2019
Historique:
received:
05
10
2018
accepted:
01
02
2019
entrez:
19
4
2019
pubmed:
19
4
2019
medline:
19
4
2019
Statut:
epublish
Résumé
The X-ray crystallography station I911-2 at MAXLab II (Lund, Sweden) has been adapted to enable difference small- and wide-angle X-ray scattering (SAXS/WAXS) data to be recorded. Modifications to the beamline included a customized flow cell, a motorized flow cell holder, a helium cone, a beam stop, a sample stage and a sample delivery system. This setup incorporated external devices such as infrared lasers, LEDs and reaction mixers to induce conformational changes in macromolecules. This platform was evaluated through proof-of-principle experiments capturing light-induced conformational changes in phytochromes. A difference WAXS signature of conformational changes in a plant aqua-porin was also demonstrated using caged calcium.
Identifiants
pubmed: 30996717
doi: 10.1107/S1600576719001900
pii: ap5035
pmc: PMC6448683
doi:
Types de publication
Journal Article
Langues
eng
Pagination
378-386Références
Biochemistry. 2002 May 28;41(21):6605-14
pubmed: 12022864
Biochemistry. 2004 Jul 20;43(28):9036-49
pubmed: 15248761
Chem Biol. 2004 Oct;11(10):1431-43
pubmed: 15489170
Nature. 2006 Feb 9;439(7077):688-94
pubmed: 16340961
J Exp Bot. 2006;57(3):609-21
pubmed: 16397000
J Biomol Screen. 2007 Oct;12(7):994-8
pubmed: 17942792
Nat Methods. 2008 Oct;5(10):881-6
pubmed: 18806790
Structure. 2009 Sep 9;17(9):1265-75
pubmed: 19748347
J Phys Chem B. 2009 Oct 8;113(40):13131-3
pubmed: 19757799
J Struct Funct Genomics. 2010 Mar;11(1):9-19
pubmed: 20049539
Proc Natl Acad Sci U S A. 2010 Apr 20;107(16):7281-6
pubmed: 20406909
Nat Methods. 2010 Oct;7(10):775-6
pubmed: 20885435
J Am Chem Soc. 2011 Jun 22;133(24):9395-404
pubmed: 21627157
Biophys J. 2011 Sep 21;101(6):1345-53
pubmed: 21943415
Rev Sci Instrum. 2012 Mar;83(3):035108
pubmed: 22462961
Curr Opin Struct Biol. 2012 Oct;22(5):651-9
pubmed: 23021004
Biophys J. 2013 Feb 19;104(4):873-83
pubmed: 23442966
Nat Commun. 2014;5:3309
pubmed: 24525480
Trends Neurosci. 1989 Feb;12(2):54-9
pubmed: 2469211
Nature. 2014 May 8;509(7499):245-248
pubmed: 24776794
Nat Methods. 2014 Sep;11(9):923-6
pubmed: 25108686
Science. 2014 Dec 5;346(6214):1242-6
pubmed: 25477465
Sci Signal. 2015 Mar 10;8(367):ra26
pubmed: 25759477
Nat Commun. 2015 Apr 02;6:6772
pubmed: 25832715
IUCrJ. 2015 Jan 27;2(Pt 2):168-76
pubmed: 25866654
J Phys Chem Lett. 2015 Sep 3;6(17):3379-83
pubmed: 26275765
Science. 2015 Oct 23;350(6259):445-50
pubmed: 26359336
Science. 2016 May 6;352(6286):725-9
pubmed: 27151871
Science. 2016 Dec 23;354(6319):1552-1557
pubmed: 28008064
Nature. 2017 Mar 2;543(7643):131-135
pubmed: 28219079
Nat Methods. 2017 Apr;14(4):443-449
pubmed: 28250468
Nat Commun. 2017 Aug 18;8(1):284
pubmed: 28819239
J Appl Crystallogr. 2017 Sep 05;50(Pt 5):1545-1553
pubmed: 29021737
Nat Chem. 2018 Jan;10(1):31-37
pubmed: 29256511
Science. 2018 Jul 13;361(6398):
pubmed: 29903883
Biophys J. 1996 Feb;70(2):1006-16
pubmed: 8789118