Randomised study of the effects of fluoride and time on in situ remineralisation of acid-softened enamel.

This single-centre, randomised, crossover study used a short-term in situ dental erosion remineralisation model to explore the remineralisation of acid-softened enamel in the 4-h period immediately following brushing with an anti-erosion, dentin hypersensitivity test dentifrice containing 1150 ppm fluoride (as sodium fluoride [NaF]) or a placebo dentifrice with no fluoride. Fifty participants wearing a palatal appliance holding surface-softened bovine enamel specimens brushed their natural teeth with their assigned dentifrice. Specimens were removed at 5-, 10-, 15-, 30-, 60-, 120- and 240-min post brushing. Enamel remineralisation effect was evaluated at each timepoint by percent surface microhardness recovery (%SMHR) and enamel fluoride uptake (EFU). After a second in vitro erosive challenge, the percent relative erosion resistance (%RER) was calculated. Statistically significant differences in %SMHR were observed for the test dentifrice compared with the placebo dentifrice from the 60-min timepoint onwards (all p < 0.02; mean difference of 8.66 [95% CI 3.46, 13.87] at 60 min). At each specimen removal time, %RER and EFU were statistically significantly higher for the test dentifrice compared with the placebo dentifrice (p < 0.0001 for all). No treatment-related or serious adverse events were reported. The NaF-containing anti-erosion, dentin hypersensitivity dentifrice improved remineralisation of acid-softened enamel starting at 60 min of intra-oral exposure. It also improved enamel erosion resistance and fluoride uptake as early as 5 min after exposure to fluoridated dentifrice slurry. Brushing with a NaF-containing dentifrice can rapidly improve remineralisation, enamel erosion resistance and fluoride uptake.