Prevalence of p53 dysregulations in feline oral squamous cell carcinoma and non-neoplastic oral mucosa.
Animals
Carcinoma, Squamous Cell
/ genetics
Cat Diseases
/ genetics
Cats
Eosinophilia
/ genetics
Gene Expression Regulation, Neoplastic
Gingival Diseases
/ genetics
Humans
Mouth Mucosa
/ metabolism
Mouth Neoplasms
/ genetics
Mutation
Prevalence
Retrospective Studies
Stomatitis
/ genetics
Tongue Diseases
/ genetics
Tumor Suppressor Protein p53
/ biosynthesis
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
09
02
2019
accepted:
04
04
2019
entrez:
19
4
2019
pubmed:
19
4
2019
medline:
8
1
2020
Statut:
epublish
Résumé
Squamous cell carcinoma is the most common malignant oral tumor in cats. The late presentation is one of the factors contributing to the detrimental prognosis of this disease. The immunohistochemical expression of the p53 tumor suppressor protein has been reported in 24% to 65% of feline oral squamous cell carcinomas, but no study has systematically evaluated in this tumor the presence of p53 encoding gene (TP53) mutations. The aim of this retrospective study was to determine whether p53 immunohistochemistry accurately reflects the mutational status of the TP53 gene in feline oral squamous cell carcinoma. Additionally, the prevalence of p53 dysregulation in feline oral squamous cell carcinoma was compared with that of feline non-neoplastic oral mucosa, in order to investigate the relevance of these dysregulations in cancer development. The association between p53 dysregulations and exposure to environmental tobacco smoke and tumor characteristics was further assessed. Twenty-six incisional biopsies of oral squamous cell carcinomas and 10 cases each of lingual eosinophilic granuloma, chronic gingivostomatitis and normal oral mucosa were included in the study. Eighteen squamous cell carcinomas (69%) expressed p53 and 18 had mutations in exons 5-8 of TP53. The agreement between immunohistochemistry and mutation analysis was 77%. None of non-neoplastic oral mucosa samples had a positive immunohistochemical staining, while one case each of eosinophilic granuloma and chronic gingivostomatitis harbored TP53 mutations. Unlike previously hypothesized, p53 dysregulations were not associated with exposure to environmental tobacco smoke. These results suggest an important role of p53 in feline oral tumorigenesis. Additionally, the immunohistochemical detection of p53 expression appears to reflect the presence of TP53 mutations in the majority of cases. It remains to be determined if the screening for p53 dysregulations, alone or in association with other markers, can eventually contribute to the early detection of this devastating disease.
Identifiants
pubmed: 30998743
doi: 10.1371/journal.pone.0215621
pii: PONE-D-19-03940
pmc: PMC6472789
doi:
Substances chimiques
Tumor Suppressor Protein p53
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0215621Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Endocr Relat Cancer. 2017 Feb;24(2):107-117
pubmed: 28062544
Patholog Res Int. 2013;2013:502197
pubmed: 23970998
Vet Sci. 2016 Aug 18;3(3):
pubmed: 29056726
Genes Dev. 1998 Oct 1;12(19):2973-83
pubmed: 9765199
Oral Oncol. 2003 Apr;39(3):222-31
pubmed: 12618194
Nat Methods. 2010 Apr;7(4):248-9
pubmed: 20354512
J Vet Dent. 2015 Spring;32(1):30-40
pubmed: 26197688
J Comp Pathol. 2012 Aug-Oct;147(2-3):111-20
pubmed: 22300705
Vet Comp Oncol. 2013 Jun;11(2):101-12
pubmed: 22443464
Vet Pathol. 2016 Jan;53(1):7-9
pubmed: 26712813
J Feline Med Surg. 2019 May;21(5):409-418
pubmed: 30477386
Vet Pathol. 1989 Mar;26(2):121-8
pubmed: 2711569
Am J Surg. 1994 Nov;168(5):429-32
pubmed: 7977967
Vet Comp Oncol. 2006 Jun;4(2):84-97
pubmed: 19754818
Vet Pathol. 2004 May;41(3):209-14
pubmed: 15133168
Head Neck Oncol. 2010 Dec 15;2:36
pubmed: 21159183
Hum Pathol. 1999 Oct;30(10):1221-5
pubmed: 10534171
N Engl J Med. 1995 Mar 16;332(11):712-7
pubmed: 7854378
Cancer Lett. 2000 Jul 3;155(1):1-7
pubmed: 10814873
Vet Pathol. 2001 Mar;38(2):236-8
pubmed: 11280384
J Small Anim Pract. 2007 Jul;48(7):394-9
pubmed: 17559521
Cell. 2017 Sep 7;170(6):1062-1078
pubmed: 28886379
J Am Vet Med Assoc. 1992 Sep 1;201(5):777-81
pubmed: 1399785
Oncotarget. 2018 Sep 4;9(69):33098-33109
pubmed: 30237854
Int J Oral Maxillofac Surg. 2007 Dec;36(12):1123-38
pubmed: 17875383
Histol Histopathol. 2001 Jan;16(1):113-21
pubmed: 11193185
J Pathol. 1998 Apr;184(4):360-8
pubmed: 9664901
Cell. 1989 Jun 30;57(7):1083-93
pubmed: 2525423
Vet Pathol. 2012 May;49(3):538-45
pubmed: 21930804
Res Vet Sci. 2000 Feb;68(1):63-70
pubmed: 10684760
J Comp Pathol. 1996 Feb;114(2):205-10
pubmed: 8920221
J Vet Intern Med. 2003 Jul-Aug;17(4):557-62
pubmed: 12892308
Cell Death Differ. 2006 Jun;13(6):941-50
pubmed: 16601750
J Pathol. 1994 Jan;172(1):5-12
pubmed: 7931827
Vet Comp Oncol. 2007 Jun;5(2):108-18
pubmed: 19754794
Biometrics. 1977 Mar;33(1):159-74
pubmed: 843571
J Cell Biochem. 2016 Dec;117(12):2682-2692
pubmed: 27166782
Lancet. 1990 Mar 24;335(8691):675-9
pubmed: 1969059