Deciphering the Nucleotide and RNA Binding Selectivity of the Mayaro Virus Macro Domain.
ADP-ribose
Alphavirus
Mayaro virus
RNA
macro domain
Journal
Journal of molecular biology
ISSN: 1089-8638
Titre abrégé: J Mol Biol
Pays: Netherlands
ID NLM: 2985088R
Informations de publication
Date de publication:
31 05 2019
31 05 2019
Historique:
received:
30
11
2018
revised:
01
03
2019
accepted:
08
04
2019
pubmed:
19
4
2019
medline:
24
3
2020
entrez:
19
4
2019
Statut:
ppublish
Résumé
Mayaro virus (MAYV) is a member of Togaviridae family, which also includes Chikungunya virus as a notorious member. MAYV recently emerged in urban areas of the Americas, and this emergence emphasized the current paucity of knowledge about its replication cycle. The macro domain (MD) of MAYV belongs to the N-terminal region of its non-structural protein 3, part of the replication complex. Here, we report the first structural and dynamical characterization of a previously unexplored Alphavirus MD investigated through high-resolution NMR spectroscopy, along with data on its ligand selectivity and binding properties. The structural analysis of MAYV MD reveals a typical "macro" (ββαββαβαβα) fold for this polypeptide, while NMR-driven interaction studies provide in-depth insights into MAYV MD-ligand adducts. NMR data in concert with thermodynamics and biochemical studies provide convincing experimental evidence for preferential binding of adenosine diphosphate ribose (ADP-r) and adenine-rich RNAs to MAYV MD, thus shedding light on the structure-function relationship of a previously unexplored viral MD. The emerging differences with any other related MD are expected to enlighten distinct functions.
Identifiants
pubmed: 30998933
pii: S0022-2836(19)30207-4
doi: 10.1016/j.jmb.2019.04.013
pmc: PMC7094482
pii:
doi:
Substances chimiques
Nucleotides
0
Viral Nonstructural Proteins
0
Adenosine Diphosphate Ribose
20762-30-5
RNA
63231-63-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2283-2297Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.
Références
Clin Infect Dis. 1999 Jan;28(1):67-73
pubmed: 10028074
J Virol. 2018 Mar 28;92(8):
pubmed: 29367248
J Biol Chem. 2014 Feb 28;289(9):5635-53
pubmed: 24407286
Mutat Res. 2011 May-Jun;727(3):86-103
pubmed: 21421074
Biomol NMR Assign. 2015 Apr;9(1):191-5
pubmed: 25217003
Crit Rev Eukaryot Gene Expr. 2014;24(1):15-28
pubmed: 24579667
Biochemistry. 2015 Feb 24;54(7):1525-33
pubmed: 25623359
J Mol Biol. 2009 Jan 9;385(1):212-25
pubmed: 18983849
PLoS One. 2011;6(7):e22336
pubmed: 21811589
J Virol. 2012 Mar;86(5):2729-38
pubmed: 22190718
Nat Rev Mol Cell Biol. 2013 Jul;14(7):443-51
pubmed: 23736681
Sci Rep. 2017 Jul 13;7(1):5343
pubmed: 28706231
J Virol. 2006 Sep;80(17):8493-502
pubmed: 16912299
An Acad Bras Cienc. 2016 Sep;88(3):1485-99
pubmed: 27627069
PLoS Negl Trop Dis. 2015 Oct 23;9(10):e0004104
pubmed: 26496497
Nucleic Acids Res. 2016 Jul 8;44(W1):W351-5
pubmed: 27131377
J Mol Biol. 2010 Jul 23;400(4):724-42
pubmed: 20493876
Prog Nucleic Acid Res Mol Biol. 2002;71:187-222
pubmed: 12102555
J Biomol NMR. 2009 Aug;44(4):213-23
pubmed: 19548092
EMBO J. 2005 Jun 1;24(11):1911-20
pubmed: 15902274
Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1666-1671
pubmed: 28143925
Structure. 2013 Mar 5;21(3):462-75
pubmed: 23473667
Acta Crystallogr D Biol Crystallogr. 2009 Dec;65(Pt 12):1292-300
pubmed: 19966415
J Mol Biol. 1997 Oct 17;273(1):283-98
pubmed: 9367762
PLoS One. 2014 Jun 24;9(6):e100531
pubmed: 24959709
J Mol Biol. 2019 Feb 15;431(4):794-806
pubmed: 30639410
PLoS Genet. 2014 May 29;10(5):e1004403
pubmed: 24875882
Travel Med Infect Dis. 2017 Jan - Feb;15:72-73
pubmed: 27826073
Infect Ecol Epidemiol. 2015 Dec 18;5:29853
pubmed: 26689654
Biochim Biophys Acta. 2011 Aug;1814(8):942-68
pubmed: 21059410
Vector Borne Zoonotic Dis. 2016 May;16(5):295-301
pubmed: 26991057
J Virol. 2017 Aug 24;91(18):
pubmed: 28701392
Structure. 2006 Sep;14(9):1449-58
pubmed: 16962975
Prog Nucl Magn Reson Spectrosc. 2013 Aug;73:1-16
pubmed: 23962882
Nat Rev Microbiol. 2010 Jul;8(7):491-500
pubmed: 20551973
Mol Cell. 2015 Jun 18;58(6):947-58
pubmed: 26091343
Viruses. 2018 Feb 28;10(3):
pubmed: 29495654
Sci Rep. 2016 Apr 11;6:24213
pubmed: 27064071
FEBS Lett. 2006 Feb 20;580(5):1502-8
pubmed: 16466719
J Virol. 2012 Aug;86(15):8147-60
pubmed: 22623789
J Virol. 2009 Jul;83(13):6534-45
pubmed: 19386706
Proc Natl Acad Sci U S A. 2018 Oct 30;115(44):E10457-E10466
pubmed: 30322911
Adv Virol. 2011;2011:249640
pubmed: 22312336
Sci Signal. 2011 May 24;4(174):mr5
pubmed: 21610250
Nucleic Acids Res. 2014 May;42(9):5993-6011
pubmed: 24682827
Protein Sci. 2009 Jan;18(1):6-16
pubmed: 19177346
EMBO J. 2013 May 2;32(9):1225-37
pubmed: 23481255
J Biomol NMR. 1995 Jul;6(1):1-10
pubmed: 22911575
Immunol Rev. 2008 Oct;225:27-45
pubmed: 18837774
Biochemistry. 2002 Jan 8;41(1):1-7
pubmed: 11771996
Nat Struct Mol Biol. 2009 Sep;16(9):923-9
pubmed: 19680243
Sci Rep. 2017 Feb 02;7:41746
pubmed: 28150709
Antiviral Res. 2017 Jul;143:38-47
pubmed: 28390873
Curr Protein Pept Sci. 2015;16(6):491-501
pubmed: 25938242
Methods Enzymol. 2011;493:241-75
pubmed: 21371594
Open Biol. 2017 Apr;7(4):
pubmed: 28446708
Curr Top Microbiol Immunol. 2015;384:3-32
pubmed: 25027823
Annu Rev Biochem. 2016 Jun 2;85:431-54
pubmed: 26844395
Biomol NMR Assign. 2018 Apr;12(1):31-35
pubmed: 28875416
Chem Rev. 2018 Feb 14;118(3):1092-1136
pubmed: 29172462
Microbes Infect. 2016 Dec;18(12):724-734
pubmed: 27989728
Genes Dev. 2017 Jan 15;31(2):101-126
pubmed: 28202539
Antiviral Res. 2018 Jan;149:58-74
pubmed: 29128390
J Virol. 2016 Sep 12;90(19):8478-86
pubmed: 27440879
Proc Natl Acad Sci U S A. 2012 Oct 9;109(41):16534-9
pubmed: 23010928