Immediate Neutrophil-Variable-T Cell Receptor Host Response in Bacterial Meningitis.

T cell receptors V(D)J receptors bacterial meningitis neutrophil granulocytes phagocytosis

Journal

Frontiers in neurology
ISSN: 1664-2295
Titre abrégé: Front Neurol
Pays: Switzerland
ID NLM: 101546899

Informations de publication

Date de publication:
2019
Historique:
received: 11 09 2018
accepted: 11 03 2019
entrez: 20 4 2019
pubmed: 20 4 2019
medline: 20 4 2019
Statut: epublish

Résumé

Bacterial meningitis is a life-threatening disease that evokes an intense neutrophil-dominated host response to microbes invading the subarachnoid space. Recent evidence indicates the existence of combinatorial V(D)J immune receptors in neutrophils that are based on the T cell receptor (TCR). Here, we investigated expression of the novel neutrophil TCRαβ-based V(D)J receptors in cerebrospinal fluid (CSF) from human patients with acute-phase bacterial meningitis using immunocytochemical, genetic immunoprofiling, cell biological, and mass spectrometric techniques. We find that the human neutrophil combinatorial V(D)J receptors are rapidly induced in CSF neutrophils during the first hours of bacterial meningitis. Immune receptor repertoire diversity is consistently increased in CSF neutrophils relative to circulating neutrophils and phagocytosis of baits directed to the variable immunoreceptor is enhanced in CSF neutrophils during acute-phase meningitis. Our results reveal that a flexible immune response involving neutrophil V(D)J receptors which enhance phagocytosis is immediately initiated at the site of acute bacterial infection.

Identifiants

pubmed: 31001192
doi: 10.3389/fneur.2019.00307
pmc: PMC6454057
doi:

Types de publication

Journal Article

Langues

eng

Pagination

307

Références

Nat Rev Neurosci. 2003 May;4(5):376-85
pubmed: 12728265
Proc Natl Acad Sci U S A. 2006 Sep 26;103(39):14441-6
pubmed: 16983085
N Engl J Med. 2006 Dec 14;355(24):2592-3; author reply 2593-4
pubmed: 17167145
Lancet. 2007 Jun 30;369(9580):2196-2210
pubmed: 17604802
Nature. 2008 Apr 10;452(7188):764-7
pubmed: 18401411
Lancet Infect Dis. 2009 Jan;9(1):31-44
pubmed: 19036641
Nat Rev Immunol. 2009 Jun;9(6):429-39
pubmed: 19461673
PLoS One. 2009 Jun 03;4(6):e5778
pubmed: 19492059
Science. 2009 Jul 3;325(5936):83-7
pubmed: 19520910
PLoS One. 2009 Jun 17;4(6):e5926
pubmed: 19536290
Science. 2009 Jul 24;325(5939):392-3
pubmed: 19628838
N Engl J Med. 2010 Jan 14;362(2):146-54
pubmed: 20071704
Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1518-23
pubmed: 20080641
Lancet Infect Dis. 2010 May;10(5):317-28
pubmed: 20417414
PLoS Pathog. 2011 Nov;7(11):e1002375
pubmed: 22114556
Biochem Biophys Res Commun. 2012 Mar 9;419(2):309-15
pubmed: 22342716
Lancet. 2012 Apr 7;379(9823):1364
pubmed: 22483032
Immunobiology. 2013 Mar;218(3):418-26
pubmed: 22749215
Aging Dis. 2012 Oct;3(5):404-13
pubmed: 23185720
J Periodontol. 2013 Oct;84(10):1493-503
pubmed: 23205919
Immunobiology. 2013 Jul;218(7):960-8
pubmed: 23312956
Cell Mol Immunol. 2014 Jan;11(1):94-104
pubmed: 24141767
Biochem Biophys Res Commun. 2015 Jan 2;456(1):59-65
pubmed: 25446098
Cell. 1989 Dec 22;59(6):1035-48
pubmed: 2598259
Oncotarget. 2015 Nov 17;6(36):39062-72
pubmed: 26429876
Immunobiology. 2017 Jan;222(1):39-44
pubmed: 26494401
Infect Immun. 2018 Jun 21;86(7):
pubmed: 29685989
PLoS One. 2018 Jun 11;13(6):e0198772
pubmed: 29889859
PLoS One. 2018 Jul 25;13(7):e0201043
pubmed: 30044851
PLoS One. 2018 Sep 21;13(9):e0204108
pubmed: 30240437
Proc Natl Acad Sci U S A. 1993 May 1;90(9):4319-23
pubmed: 8483950
J Exp Med. 1998 Apr 6;187(7):1019-28
pubmed: 9529318

Auteurs

Tina Fuchs (T)

Institute for Clinical Chemistry, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.

Kerstin Puellmann (K)

Aesculabor Hamburg, Hamburg, Germany.

David H Dreyfus (DH)

Department of Pediatrics, Yale School of Medicine, New Haven, CT, United States.

Armin P Piehler (AP)

Bioscientia Institute for Medical Diagnostics, Karlsfeld, Germany.

Björn Reuter (B)

Department of Neurology, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.

Christopher Schwarzbach (C)

Department of Neurology, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.

Olaf Willmann (O)

Department of Neurology, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.

Diego Yepes (D)

Institute for Clinical Chemistry, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.

Victor Costina (V)

Institute for Clinical Chemistry, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.

Peter Findeisen (P)

Institute for Clinical Chemistry, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.

Jens Mahrt (J)

Molecular & Optical Live Cell Imaging, University of Göttingen, Göttingen, Germany.

Chunlin Wang (C)

iRepertoire Inc., Huntsville, AL, United States.

Jian Han (J)

iRepertoire Inc., Huntsville, AL, United States.
HudsonAlpha Institute for Biotechnology, Huntsville, AL, United States.

Alexander W Beham (AW)

Department of Surgery, Hospital of Siegen, Siegen, Germany.

Michael Neumaier (M)

Institute for Clinical Chemistry, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.

Wolfgang E Kaminski (WE)

Bioscientia Institute for Medical Diagnostics, Ingelheim, Germany.

Classifications MeSH