Development of a Transplantable GFP+ B-Cell Lymphoma Tumor Cell Line From MHC-Defined Miniature Swine: Potential for a Large Animal Tumor Model.

GFP+ tumor cell line MHC defined miniature swine large animal tumor model swine lymphoma tumor cell line transplantable swine tumors

Journal

Frontiers in oncology
ISSN: 2234-943X
Titre abrégé: Front Oncol
Pays: Switzerland
ID NLM: 101568867

Informations de publication

Date de publication:
2019
Historique:
received: 27 12 2018
accepted: 11 03 2019
entrez: 20 4 2019
pubmed: 20 4 2019
medline: 20 4 2019
Statut: epublish

Résumé

The lack of a reliable and reproducible large animal tumor model for the study of hemolymphatic malignancies limits the ability to explore the underlying pathophysiology and testing of novel therapies. The goal of this study was to develop an aggressive, trackable swine tumor cell line in mice for adoptive transfer into MHC matched swine. Two tumor cell lines, post-transplant lymphoproliferative disease (PTLD) 13271 and chronic myelogenous leukemia (CML) 14736, were previously established from the Massachusetts General Hospital (MGH) miniature swine herd. PTLD 13271 is a swine B-cell lymphoma line originating from an animal that developed PTLD following hematopoietic cell transplantation (HCT), while CML 14736 was generated from a swine that spontaneously developed CML. In order to select for aggressive tumor variants, both lines were passage into NOD/SCID IL-2 receptor γ

Identifiants

pubmed: 31001475
doi: 10.3389/fonc.2019.00209
pmc: PMC6454861
doi:

Types de publication

Journal Article

Langues

eng

Pagination

209

Subventions

Organisme : NCRR NIH HHS
ID : K01 RR024466
Pays : United States

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Auteurs

Marian Schenk (M)

Center for Transplantation Sciences, Massachusetts General Hospital, Charlestown, MA, United States.

Abraham J Matar (AJ)

Center for Transplantation Sciences, Massachusetts General Hospital, Charlestown, MA, United States.
Department of Surgery, Emory University, Atlanta, GA, United States.

Isabel Hanekamp (I)

Center for Transplantation Sciences, Massachusetts General Hospital, Charlestown, MA, United States.

Robert J Hawley (RJ)

Center for Transplantation Sciences, Massachusetts General Hospital, Charlestown, MA, United States.

Christene A Huang (CA)

Center for Transplantation Sciences, Massachusetts General Hospital, Charlestown, MA, United States.
Department of Surgery, University of Colorado-Denver, Denver, CO, United States.

Raimon Duran-Struuck (R)

Center for Transplantation Sciences, Massachusetts General Hospital, Charlestown, MA, United States.
Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, United States.

Classifications MeSH