Unifying photocycle model for light adaptation and temporal evolution of cation conductance in channelrhodopsin-2.
channelrhodopsin-2
electrophysiology
optogenetics
photoisomerization
time-resolved FTIR
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
07 05 2019
07 05 2019
Historique:
pubmed:
21
4
2019
medline:
27
3
2020
entrez:
21
4
2019
Statut:
ppublish
Résumé
Although channelrhodopsin (ChR) is a widely applied light-activated ion channel, important properties such as light adaptation, photocurrent inactivation, and alteration of the ion selectivity during continuous illumination are not well understood from a molecular perspective. Herein, we address these open questions using single-turnover electrophysiology, time-resolved step-scan FTIR, and Raman spectroscopy of fully dark-adapted ChR2. This yields a unifying parallel photocycle model integrating now all so far controversial discussed data. In dark-adapted ChR2, the protonated retinal Schiff base chromophore (RSBH
Identifiants
pubmed: 31004059
pii: 1818707116
doi: 10.1073/pnas.1818707116
pmc: PMC6510988
doi:
Substances chimiques
Cations
0
Channelrhodopsins
0
Protons
0
Retinaldehyde
RR725D715M
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9380-9389Informations de copyright
Copyright © 2019 the Author(s). Published by PNAS.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
J Bioenerg Biomembr. 1992 Apr;24(2):169-79
pubmed: 1326515
Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13940-5
pubmed: 14615590
Nat Neurosci. 2005 Sep;8(9):1263-8
pubmed: 16116447
Biophys J. 2005 Dec;89(6):3911-8
pubmed: 16169986
Nature. 2006 Jan 5;439(7072):109-12
pubmed: 16280982
EMBO J. 1986 Apr;5(4):805-11
pubmed: 16453681
Proc Natl Acad Sci U S A. 1984 Apr;81(7):2055-9
pubmed: 16593445
Biophys J. 2006 Dec 15;91(12):4519-27
pubmed: 17012323
J Am Chem Soc. 2007 Jul 11;129(27):8644-9
pubmed: 17569538
J Mol Biol. 2008 Jan 18;375(3):686-94
pubmed: 18037436
J Biol Chem. 2008 Dec 12;283(50):35033-41
pubmed: 18927082
Photochem Photobiol. 2009 Jan-Feb;85(1):400-11
pubmed: 19161406
Biophys J. 2009 Mar 4;96(5):1803-14
pubmed: 19254539
J Am Chem Soc. 2009 Jun 3;131(21):7313-9
pubmed: 19422231
J Phys Chem B. 2009 Jul 30;113(30):10496-508
pubmed: 19580300
Proc Natl Acad Sci U S A. 2009 Jul 28;106(30):12317-22
pubmed: 19590013
Biochemistry. 2010 Jan 19;49(2):267-78
pubmed: 20000562
Biophys J. 2010 Mar 3;98(5):753-61
pubmed: 20197028
Chemphyschem. 2010 Apr 26;11(6):1120-6
pubmed: 20349494
Chemphyschem. 2010 Oct 4;11(14):3113-22
pubmed: 20730849
Proc Natl Acad Sci U S A. 2011 May 3;108(18):7595-600
pubmed: 21504945
Biochem Biophys Res Commun. 2011 Jul 15;410(4):737-43
pubmed: 21683688
Cell. 2011 Dec 23;147(7):1446-57
pubmed: 22196724
J Biol Chem. 2012 Feb 24;287(9):6904-11
pubmed: 22219197
Nature. 2012 Jan 22;482(7385):369-74
pubmed: 22266941
J Biol Chem. 2013 Apr 12;288(15):10451-8
pubmed: 23439646
Biophys J. 2013 Feb 19;104(4):807-17
pubmed: 23442959
Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):E1273-81
pubmed: 23509282
Biochim Biophys Acta. 2014 May;1837(5):606-13
pubmed: 24055285
Science. 2014 Apr 25;344(6182):409-12
pubmed: 24674867
Proc Natl Acad Sci U S A. 1989 Jul;86(13):4943-7
pubmed: 2544884
J Chem Phys. 2014 Dec 14;141(22):22D507
pubmed: 25494778
Angew Chem Int Ed Engl. 2015 Apr 13;54(16):4953-7
pubmed: 25537168
Sci Rep. 2015 May 08;5:10320
pubmed: 25955978
Proc Natl Acad Sci U S A. 2015 Aug 11;112(32):9896-901
pubmed: 26216996
Biochemistry. 2015 Sep 8;54(35):5389-400
pubmed: 26237332
Sci Signal. 2015 Aug 11;8(389):rs8
pubmed: 26268609
Proc Natl Acad Sci U S A. 2015 Oct 27;112(43):E5796-804
pubmed: 26460012
Biophys J. 1989 Oct;56(4):693-706
pubmed: 2819234
Angew Chem Int Ed Engl. 2017 Mar 27;56(14):3842-3846
pubmed: 28251753
J Vis Exp. 2017 May 22;(123):
pubmed: 28570519
J Biol Chem. 2017 Aug 25;292(34):14205-14216
pubmed: 28659342
Sci Rep. 2017 Aug 30;7(1):9928
pubmed: 28855540
Science. 2017 Nov 24;358(6366):
pubmed: 29170206
Science. 2017 Nov 24;358(6366):1000-1001
pubmed: 29170223
Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):3557-3562
pubmed: 29555736
J Am Chem Soc. 2018 Aug 8;140(31):9899-9903
pubmed: 30036055
Nat Commun. 2018 Sep 26;9(1):3949
pubmed: 30258177
Biophys J. 1993 Nov;65(5):1929-41
pubmed: 8298022
Biophys Struct Mech. 1977 Jun 29;3(2):79-94
pubmed: 890059