CRISPR in Parasitology: Not Exactly Cut and Dried!
Anopheles
CRISPR
CRISPRi
Leishmania
Plasmodium
Trypanosoma
Journal
Trends in parasitology
ISSN: 1471-5007
Titre abrégé: Trends Parasitol
Pays: England
ID NLM: 100966034
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
08
02
2019
revised:
13
03
2019
accepted:
14
03
2019
pubmed:
23
4
2019
medline:
28
5
2020
entrez:
23
4
2019
Statut:
ppublish
Résumé
CRISPR/Cas9 technology has been developing rapidly in the field of parasitology, allowing for the dissection of molecular processes with unprecedented efficiency. Optimization and implementation of a new technology like CRISPR, especially in nonmodel organisms, requires communication and collaboration throughout the field. Recently, a 'CRISPR in Parasitology' symposium was held at the Institut Pasteur Paris, bringing together scientists studying Leishmania, Plasmodium, Trypanosoma, and Anopheles. Here we share technological advances and challenges in using CRISPR/Cas9 in the parasite and vector systems that were discussed. As CRISPR/Cas9 continues to be applied to diverse parasite systems, the community should now focus on improvement and standardization of the technique as well as expanding the CRISPR toolkit to include Cas9 alternatives/derivatives for more advanced applications like genome-wide functional screens.
Identifiants
pubmed: 31006600
pii: S1471-4922(19)30055-8
doi: 10.1016/j.pt.2019.03.004
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
409-422Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.