Layer-specific arterial micromechanics and microstructure: Influences of age, anatomical location, and processing technique.
Atomic force microscopy
Collagen
Elastin
Glycosaminoglycans
Multiphoton microscopy
Journal
Journal of biomechanics
ISSN: 1873-2380
Titre abrégé: J Biomech
Pays: United States
ID NLM: 0157375
Informations de publication
Date de publication:
09 May 2019
09 May 2019
Historique:
received:
07
09
2018
revised:
24
02
2019
accepted:
18
03
2019
pubmed:
24
4
2019
medline:
7
7
2020
entrez:
24
4
2019
Statut:
ppublish
Résumé
The importance of matrix micromechanics is increasingly recognized in cardiovascular research due to the intimate role they play in local vascular cell physiology. However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown. This study determined layer-specific stiffness in elastic arteries, including the main pulmonary artery, ascending aorta, and carotid artery using atomic force indentation. To compare stiffness with age and frozen processing techniques, neonatal and adult pulmonary arteries were tested, while fresh (vibratomed) and frozen (cryotomed) tissues were tested from the adult aorta. Results revealed that the mean compressive modulus varied among the intima, sub-luminal media, inner-middle media, and adventitia layers in the range of 1-10 kPa for adult arteries. Adult samples, when compared to neonatal pulmonary arteries, exhibited increased stiffness in all layers except adventitia. Compared to freshly isolated samples, frozen preparation yielded small stiffness increases in each layer to varied degrees, thus inaccurately representing physiological stiffness. To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence. Composition analysis of matrix protein area density demonstrated that decrease in the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios corresponded to stiffness increases in identical layers among different types of arteries. However, composition analysis was insufficient to interpret stiffness variations between layers which had dissimilar microstructure. Detailed microstructure analyses may contribute to more complete understanding of arterial micromechanics.
Identifiants
pubmed: 31010593
pii: S0021-9290(19)30206-4
doi: 10.1016/j.jbiomech.2019.03.026
pmc: PMC6499687
mid: NIHMS1526129
pii:
doi:
Substances chimiques
Glycosaminoglycans
0
Collagen
9007-34-5
Elastin
9007-58-3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
113-121Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL119371
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.
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