Modification and Clinical Application of the Inner Perivitelline Membrane Test in Different Avian Species.
assisted reproduction
bird
functional sperm-assay
perivitelline-membrane-penetration
Journal
Veterinary sciences
ISSN: 2306-7381
Titre abrégé: Vet Sci
Pays: Switzerland
ID NLM: 101680127
Informations de publication
Date de publication:
12 Apr 2019
12 Apr 2019
Historique:
received:
26
02
2019
revised:
29
03
2019
accepted:
10
04
2019
entrez:
25
4
2019
pubmed:
25
4
2019
medline:
25
4
2019
Statut:
epublish
Résumé
The aim of this study was to adapt an inner perivitelline membrane (IPVM) test as an interspecies penetration assay for avian spermatozoa. The IPVM of different bird species was evaluated to test the penetrating ability of avian spermatozoa in an intra- and interspecies design. Isolation of the IPVM via acid hydrolysis was tested in pre-incubated chicken eggs and in six other avian species. The separation protocol was modified (time, acid concentration) to facilitate practicability. Separated membranes were evaluated with dark field microscopy for the presence of holes produced by penetrating spermatozoa. In chicken eggs, the influence of different membrane storage conditions was tested. In the penetration assay, the IPVM of chicken eggs was used as a model for fresh and frozen-thawed rooster sperm and for fresh spermatozoa of cockatiels and falcons. Results demonstrated that the time of egg-incubation had a significantly negative influence on the isolation ability of the IPVM (
Identifiants
pubmed: 31013715
pii: vetsci6020039
doi: 10.3390/vetsci6020039
pmc: PMC6631061
pii:
doi:
Types de publication
Journal Article
Langues
eng
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