Priming Dental Pulp Stem Cells from Human Exfoliated Deciduous Teeth with Fibroblast Growth Factor-2 Enhances Mineralization Within Tissue-Engineered Constructs Implanted in Craniofacial Bone Defects.
Animals
Bone Regeneration
Calcification, Physiologic
Cells, Cultured
Child
Child, Preschool
Collagen
/ chemistry
Dental Pulp
/ cytology
Female
Fibroblast Growth Factor 2
/ pharmacology
Humans
Hydrogels
/ chemistry
Male
Mesenchymal Stem Cells
/ cytology
Mice
Mice, Nude
Skull
/ injuries
Tissue Engineering
/ methods
Tissue Scaffolds
/ chemistry
Tooth, Deciduous
/ cytology
Bone engineering
Calvaria
Hypoxia
Intramembranous ossification
Mesenchymal stem cells
Journal
Stem cells translational medicine
ISSN: 2157-6580
Titre abrégé: Stem Cells Transl Med
Pays: England
ID NLM: 101578022
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
20
08
2018
accepted:
03
12
2018
pubmed:
25
4
2019
medline:
4
6
2020
entrez:
25
4
2019
Statut:
ppublish
Résumé
The craniofacial area is prone to trauma or pathologies often resulting in large bone damages. One potential treatment option is the grafting of a tissue-engineered construct seeded with adult mesenchymal stem cells (MSCs). The dental pulp appears as a relevant source of MSCs, as dental pulp stem cells display strong osteogenic properties and are efficient at bone formation and repair. Fibroblast growth factor-2 (FGF-2) and/or hypoxia primings were shown to boost the angiogenesis potential of dental pulp stem cells from human exfoliated deciduous teeth (SHED). Based on these findings, we hypothesized here that these primings would also improve bone formation in the context of craniofacial bone repair. We found that both hypoxic and FGF-2 primings enhanced SHED proliferation and osteogenic differentiation into plastically compressed collagen hydrogels, with a much stronger effect observed with the FGF-2 priming. After implantation in immunodeficient mice, the tissue-engineered constructs seeded with FGF-2 primed SHED mediated faster intramembranous bone formation into critical size calvarial defects than the other groups (no priming and hypoxia priming). The results of this study highlight the interest of FGF-2 priming in tissue engineering for craniofacial bone repair. Stem Cells Translational Medicine 2019;8:844&857.
Identifiants
pubmed: 31016898
doi: 10.1002/sctm.18-0182
pmc: PMC6646701
doi:
Substances chimiques
Hydrogels
0
Fibroblast Growth Factor 2
103107-01-3
Collagen
9007-34-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
844-857Informations de copyright
© 2019 The Authors. Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press.
Références
J Cell Biol. 2000 Jun 12;149(6):1297-308
pubmed: 10851026
J Bone Miner Res. 1993 Apr;8(4):485-96
pubmed: 8475798
Soft Matter. 2006 Oct 17;2(11):986-992
pubmed: 32680187
J Clin Invest. 2000 Apr;105(8):1085-93
pubmed: 10772653
J Dent. 2014 Jul;42(7):761-8
pubmed: 24589847
Cell Transplant. 2011;20(7):1003-13
pubmed: 21054950
J Tissue Eng Regen Med. 2015 Nov;9(11):1205-16
pubmed: 24850632
Eur J Oral Implantol. 2014 Summer;7 Suppl 2:S203-17
pubmed: 24977256
Oral Dis. 2008 Jul;14(5):428-34
pubmed: 18938268
J Periodontal Res. 2009 Dec;44(6):794-802
pubmed: 19602122
Sci Transl Med. 2018 Aug 22;10(455):
pubmed: 30135248
J Dent Res. 2013 Jun;92(6):524-31
pubmed: 23609159
Adv Drug Deliv Rev. 2013 Apr;65(4):429-56
pubmed: 22960357
J Dent Res. 2009 Mar;88(3):249-54
pubmed: 19329459
Cell. 2011 Sep 16;146(6):873-87
pubmed: 21925313
Differentiation. 2009 Sep-Oct;78(2-3):79-90
pubmed: 19433344
Sci Rep. 2016 Dec 09;6:38814
pubmed: 27934940
Bone. 2009 Apr;44(4):699-707
pubmed: 19166987
Int J Oral Maxillofac Implants. 2014 Jan-Feb;29(1):e122-9
pubmed: 24451880
Cell Death Differ. 2007 Jun;14(6):1162-71
pubmed: 17347663
Int J Oral Maxillofac Implants. 2006 Sep-Oct;21(5):711-8
pubmed: 17066631
Acta Orthop Belg. 2007 Oct;73(5):551-7
pubmed: 18019908
World J Stem Cells. 2014 Sep 26;6(4):505-10
pubmed: 25258673
Bone. 2004 Mar;34(3):402-11
pubmed: 15003788
J Craniofac Surg. 2017 Jul;28(5):1136-1141
pubmed: 28665863
J Tissue Eng. 2018 Jan 17;9:2041731417752766
pubmed: 29375756
Stem Cells. 2008 Sep;26(9):2408-18
pubmed: 18583536
BMB Rep. 2008 Dec 31;41(12):833-9
pubmed: 19123972
J Dent Res. 2013 Jul;92(7):648-54
pubmed: 23632809
Stem Cell Res Ther. 2018 Apr 11;9(1):100
pubmed: 29642953
J Biomed Mater Res B Appl Biomater. 2014 May;102(4):815-25
pubmed: 24142538
J Dent Res. 2013 Jan;92(1):51-7
pubmed: 23114032
Stem Cells Transl Med. 2016 Mar;5(3):392-404
pubmed: 26798059
Biomacromolecules. 2011 Aug 8;12(8):2946-56
pubmed: 21661759
Biomed Res Int. 2014;2014:865270
pubmed: 24804256
Proc Natl Acad Sci U S A. 2003 May 13;100(10):5807-12
pubmed: 12716973
J Endod. 2011 Dec;37(12):1647-52
pubmed: 22099898
Cell Mol Life Sci. 2008 Jan;65(1):177-86
pubmed: 17994289
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1274-8
pubmed: 286310
Adv Drug Deliv Rev. 2011 Apr 30;63(4-5):300-11
pubmed: 21396416
Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13625-30
pubmed: 11087820
Stem Cells Int. 2015;2015:378368
pubmed: 26106427
PLoS One. 2012;7(2):e31771
pubmed: 22359627
Tissue Eng Part B Rev. 2012 Apr;18(2):129-38
pubmed: 22032258
Biomaterials. 2013 Mar;34(8):1888-97
pubmed: 23245334
J Bone Miner Res. 2010 Jul;25(7):1468-86
pubmed: 20533309
J Dent Res. 2017 Feb;96(2):137-144
pubmed: 28106505
J Orthop Res. 2002 Sep;20(5):1091-8
pubmed: 12382977
Cell Cycle. 2008 Apr 15;7(8):1013-9
pubmed: 18414040
Tissue Eng Part A. 2010 Mar;16(3):781-93
pubmed: 19778181
J Orthop Res. 2012 Feb;30(2):260-6
pubmed: 21809383
Tissue Eng Part A. 2016 Mar;22(5-6):556-67
pubmed: 26896424
Stem Cells Int. 2017;2017:2374161
pubmed: 29230248
Clin Orthop Relat Res. 2000 Feb;(371):10-27
pubmed: 10693546
J Dent Res. 2018 Feb;97(2):184-191
pubmed: 28880715
J Oral Maxillofac Surg. 2007 Oct;65(10):2039-46
pubmed: 17884536
PLoS One. 2013 Aug 07;8(8):e71104
pubmed: 23951091
J Endod. 2012 Apr;38(4):454-63
pubmed: 22414829
Tissue Eng Part A. 2016 Oct;22(19-20):1191-1203
pubmed: 27586636
J Biol Chem. 2003 Aug 22;278(34):32005-13
pubmed: 12788918
Stem Cells Dev. 2015 Jul 15;24(14):1610-22
pubmed: 25869156
Biomater Sci. 2015 Feb;3(2):373-82
pubmed: 26218128
Acta Biomater. 2018 Dec;82:111-121
pubmed: 30312778