Probing the Cellular Size Distribution in Cell Samples Undergoing Cell Death.
Cell death
Polydispersity
Quantitative ultrasound
Scatterer size distribution
Structure factor model
Journal
Ultrasound in medicine & biology
ISSN: 1879-291X
Titre abrégé: Ultrasound Med Biol
Pays: England
ID NLM: 0410553
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
01
05
2018
revised:
06
01
2019
accepted:
07
01
2019
pubmed:
27
4
2019
medline:
13
2
2020
entrez:
27
4
2019
Statut:
ppublish
Résumé
A polydisperse scattering model adapted for concentrated medium, namely the polydisperse structure factor model, was examined to explain the backscatter coefficients (BSCs) measured from packed cell samples undergoing cell death. Cell samples were scanned using high-frequency ultrasound in the 10-42 MHz bandwidth. A parameter estimation procedure was proposed to estimate the volume fraction and the relative impedance contrast that could explain the changes in BSC pattern by considering the actual change in cellular size distribution. Quantitative ultrasound parameters were estimated and related to the percentage of dead cells determined by flow cytometry. The standard deviation of scatterer size distribution extracted from the polydisperse structure factor model and the spectral intercept were found to be strongly correlated to the percentage of dead cells (r
Identifiants
pubmed: 31023498
pii: S0301-5629(19)30015-8
doi: 10.1016/j.ultrasmedbio.2019.01.006
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1787-1798Informations de copyright
Copyright © 2019 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.