Tetramethylrhodamine is an essential scaffold of azide probe in detecting cellular acrolein.
1,3-Dipolar cycloaddition
Acrolein
Breast cancer cell lines
Phenyl azide
TAMRA
Journal
Bioorganic & medicinal chemistry
ISSN: 1464-3391
Titre abrégé: Bioorg Med Chem
Pays: England
ID NLM: 9413298
Informations de publication
Date de publication:
01 06 2019
01 06 2019
Historique:
received:
22
03
2019
revised:
15
04
2019
accepted:
17
04
2019
pubmed:
27
4
2019
medline:
23
9
2020
entrez:
27
4
2019
Statut:
ppublish
Résumé
Tetramethylrhodamine (TAMRA)-phenyl azide is a chemical probe used to detect intracellular acrolein directly in live cells. Herein, we demonstrated that TAMRA is the optimum fluorophore for the probe. TAMRA-phenyl azide was used to reveal that high levels of acrolein are generated in a variety of breast cancer cells, regardless of the tumor subtype. These findings corroborate the analysis presented in our previous report, in which TAMRA-phenyl azide was used to label breast cancer tissues resected from breast cancer patients. Because high levels of acrolein were generated in all cancer cell types, we believe that acrolein detection may be useful as a general method for labeling cancerous tissues.
Identifiants
pubmed: 31023557
pii: S0968-0896(19)30478-X
doi: 10.1016/j.bmc.2019.04.026
pii:
doi:
Substances chimiques
Azides
0
Biomarkers
0
Fluorescent Dyes
0
Rhodamines
0
tetramethylrhodamine
62669-72-1
Acrolein
7864XYD3JJ
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2228-2234Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.