Buforin-1 blocks neuronal SNARE-mediated membrane fusion by inhibiting SNARE complex assembly.
Buforin-1
Membrane fusion
Neuronal SNARE
Neurotransmission
Synaptic vesicle
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
18 06 2019
18 06 2019
Historique:
received:
13
04
2019
accepted:
17
04
2019
pubmed:
28
4
2019
medline:
27
6
2020
entrez:
28
4
2019
Statut:
ppublish
Résumé
Assembly of neuronal SNARE protein complexes is essential for fusion of synaptic vesicles with the presynaptic plasma membrane, which releases neurotransmitters into the synaptic cleft and mediates neurotransmission. However, despite the potential of pharmacological regulation of this process for the treatment of various neurological disorders, only a few reagents, including botulinum neurotoxins, are currently available. Here, we report that buforin-1, an antimicrobial peptide from the Asian toad Bufo gargarizans, inhibits neuronal SNARE complex assembly, resulting in neuronal SNARE-mediated membrane fusion in vitro via its direct association with neuronal t-SNAREs syntaxin-1 and SNAP-25. Consistently, buforin-1 significantly inhibited neuronal-SNARE-mediated exocytosis in PC-12 cells. Thus, buforin-1 has potential for the treatment of neurological disorders caused by dysregulated neurotransmission.
Identifiants
pubmed: 31027731
pii: S0006-291X(19)30774-0
doi: 10.1016/j.bbrc.2019.04.124
pii:
doi:
Substances chimiques
Proteins
0
SNARE Proteins
0
Synaptosomal-Associated Protein 25
0
Syntaxin 1
0
buforin I
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
105-111Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.