Single-Cell Live Imaging.
Animals
Cell Culture Techniques
/ methods
Cell Survival
Cells, Cultured
Equipment Design
Fluorescence Resonance Energy Transfer
/ instrumentation
Fluorescent Dyes
/ analysis
Imaging, Three-Dimensional
/ instrumentation
Mice
Microscopy, Fluorescence
/ instrumentation
Optical Imaging
/ instrumentation
Single-Cell Analysis
/ instrumentation
Whole Body Imaging
/ instrumentation
2D/3D-cultured cell observation
Fluorescent probes
Living mouse observation
Microscopy
Single cell imaging
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
28
4
2019
pubmed:
28
4
2019
medline:
31
8
2019
Statut:
ppublish
Résumé
Recent fluorescence microscopy allows for high-throughput acquisition of 5D (X, Y, Z, T, and Color) images in various targets such as cultured cells, 3D spheroid/organoid, and even living tissue with single-cell resolution. The technology is considered promising to augment insights on heterogeneous features of both physiological and pathological cell phenotypes, for instance, distinct responses of cancer cells to anticancer drug treatment. Here we overview microscopic applications to capture live cell events for different types of targets, together with a couple of proof of concepts. The 2D live imaging will be exemplified by a FRET-based time-lapse cultured cell imaging, and 3D tissue imaging protocol will be complemented with a method for mouse skin live imaging.
Identifiants
pubmed: 31028651
doi: 10.1007/978-1-4939-9240-9_24
doi:
Substances chimiques
Fluorescent Dyes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM