Reproducibility of positive results for rare pathogens on the FilmArray GI Panel.
Adolescent
Adult
Aged
Aged, 80 and over
Animals
Bacteria
/ genetics
Child
Child, Preschool
False Positive Reactions
Feces
/ microbiology
Female
Fungi
/ genetics
Gastroenteritis
/ diagnosis
Humans
Infant
Male
Middle Aged
Molecular Diagnostic Techniques
/ methods
Multiplex Polymerase Chain Reaction
/ standards
Parasites
/ genetics
Reproducibility of Results
Sensitivity and Specificity
Young Adult
Diagnostic stewardship
FilmArray GI Panel
Molecular diagnostics
Reproducibility
Journal
Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
23
01
2019
revised:
23
03
2019
accepted:
24
03
2019
pubmed:
29
4
2019
medline:
18
12
2019
entrez:
29
4
2019
Statut:
ppublish
Résumé
Though the FilmArray GI Panel has a reported aggregate specificity and reproducibility of >97% and > 99%, respectively, the reproducibility is less understood in clinical practice. We measured the reproducibility of positive results for low-prevalence pathogens. Samples with positive results for selected targets were repeated using a different FilmArray module. Overall, 331 of 373 (89%) results were reproducible. Giardia lamblia (57/57, 100%), Cryptosporidium spp. (61/63, 97%), Cyclospora cayetanensis (34/35, 97%), Plesiomonas shigelloides (17/18, 94%), and Rotavirus A (76/77, 99%) were highly reproducible, while Adenovirus F40/41 (38/54, 70%), Vibrio spp. (8/10, 80%), V. cholerae (3/8, 37.5%), and Yersinia enterocolitica (36/50, 72%) were poorly reproducible. Review of 38 patients with nonreproducible results showed that 19 (50%) had evidence of gastroenteritis and only 6 (16%) had possible infection with the organism that showed a nonreproducible result. Higher false-positive rates with certain targets on FAGP emphasize the need for diagnostic stewardship.
Identifiants
pubmed: 31029490
pii: S0732-8893(19)30086-0
doi: 10.1016/j.diagmicrobio.2019.03.013
pmc: PMC7286436
mid: NIHMS1596199
pii:
doi:
Types de publication
Journal Article
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
10-14Subventions
Organisme : NIAID NIH HHS
ID : T32 AI007502
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI052073
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.
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