High-content analysis of particulate matters-induced oxidative stress and organelle dysfunction in vitro.
Cell Line
Endoplasmic Reticulum
/ drug effects
Endothelial Cells
/ drug effects
Epithelial Cells
/ drug effects
Fibroblasts
/ drug effects
High-Throughput Screening Assays
Humans
Lysosomes
/ drug effects
Mitochondria
/ drug effects
Organelles
/ drug effects
Oxidative Stress
/ drug effects
Particulate Matter
/ toxicity
Vehicle Emissions
/ toxicity
Carbon black
Diesel exhaust particle
High-content screening
Organelle dysfunction
Oxidative stress
Particulate matter
Journal
Toxicology in vitro : an international journal published in association with BIBRA
ISSN: 1879-3177
Titre abrégé: Toxicol In Vitro
Pays: England
ID NLM: 8712158
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
13
02
2019
revised:
19
04
2019
accepted:
24
04
2019
pubmed:
29
4
2019
medline:
24
12
2019
entrez:
29
4
2019
Statut:
ppublish
Résumé
Oxidative stress is usually considered to be a common mechanism by which particulate matter (PM) exposure induces adverse effects. However, the further biological events such as organelle dysfunction following oxidative stress remain to be explored. In this study, we applied high-content screening (HCS) technique to investigate the toxicological effects of carbon black (CB), diesel exhaust particle (DEP) and PM2.5 on oxidative stress and organelle function in human bronchial epithelial cell (16HBE), human embryo lung fibroblast cell (HELF) and human umbilical vein endothelial cell (HUVEC) which were used to represent distinct regions of the lung, and compared the toxicity impacts of different PMs and the sensitiveness of cell lines. We found three types of PMs induced mitochondrial dysfunction in three cell lines and lysosomal alkalinization in HUVEC while only CB triggered endoplasmic reticulum (ER) stress in 16HBE and HUVEC, and oxidative stress might mediate these processes. Moreover, CB basically exhibited more potent toxicity compared with DEP and PM2.5, which might be attributed to its less oxygen content. Finally, the finding that PMs-induced toxicity impacts exhibited a cell-type dependent manner might provide some information to help to understand the sensitivity of different tissue in the lung.
Identifiants
pubmed: 31029784
pii: S0887-2333(19)30128-6
doi: 10.1016/j.tiv.2019.04.026
pii:
doi:
Substances chimiques
Particulate Matter
0
Vehicle Emissions
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
263-274Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.