Online measurement of oxygen enables continuous noninvasive evaluation of human-induced pluripotent stem cell (hiPSC) culture in a perfused 3D hollow-fiber bioreactor.
biomedical research
bioreactors
cell culture
induced pluripotent stem cells
oxygen
Journal
Journal of tissue engineering and regenerative medicine
ISSN: 1932-7005
Titre abrégé: J Tissue Eng Regen Med
Pays: England
ID NLM: 101308490
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
22
10
2018
revised:
28
03
2019
accepted:
17
04
2019
pubmed:
30
4
2019
medline:
12
6
2020
entrez:
30
4
2019
Statut:
ppublish
Résumé
For clinical and/or pharmaceutical use of human-induced pluripotent stem cells (hiPSCs), large cell quantities of high quality are demanded. Therefore, we combined the expansion of hiPSCs in closed, perfusion-based 3D bioreactors with noninvasive online monitoring of oxygen as culture control mechanism. Bioreactors with a cell compartment volume of 3 or 17 ml were inoculated with either 10 × 10
Substances chimiques
Oxygen
S88TT14065
Types de publication
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1203-1216Informations de copyright
© 2019 John Wiley & Sons, Ltd.