Changes in the Proliferation Rate, Clonogenicity, and Radiosensitivity of Cultured Cells During and After Continuous Low-Dose-Rate Irradiation.
adaptive response
double strand break (DSB)
hormesis
low-dose radiation
γH2AX
Journal
Dose-response : a publication of International Hormesis Society
ISSN: 1559-3258
Titre abrégé: Dose Response
Pays: United States
ID NLM: 101308899
Informations de publication
Date de publication:
Historique:
received:
29
11
2018
revised:
04
03
2019
accepted:
17
03
2019
entrez:
2
5
2019
pubmed:
2
5
2019
medline:
2
5
2019
Statut:
epublish
Résumé
We investigated the effects of continuous low-dose radiation on proliferation, clonogenicity, radiosensitivity, and repair of DNA double-strand breaks (DSBs) in human salivary gland (HSG) tumor cells. Human salivary gland cells were cultured on acrylic boards above very-low-dose (4.3 μSv/h) or low-dose (27 μSv/h) radiation-emitting sheets or without sheets. Total cell numbers and plating efficiencies were compared among the 3 groups every 1 or 2 weeks until 6 weeks after starting culture. At 2, 4, and 6 weeks, surviving fractions of HSG cells after irradiation at 2 to 8 Gy cultured on the very-low-dose or low-dose sheets were compared to those of the control. At 4 weeks, HSG cells irradiated at 2 Gy were assessed for phosphorylated histone (γH2AX) foci formation, and DSBs were evaluated. No significant differences were observed in total cell number or plating efficiencies with or without low-dose-emitting sheets. The surviving fractions after irradiation of the very-low-dose group at 2 to 6 weeks and those of the low-dose group at 2 to 4 weeks were higher than those of the control (
Identifiants
pubmed: 31040760
doi: 10.1177/1559325819842733
pii: 10.1177_1559325819842733
pmc: PMC6477768
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1559325819842733Déclaration de conflit d'intérêts
Declaration of Conflicting Interests: The author(s) declare no potential conflicts of interest with respect to the research, authorship, or publication of this article.
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