Sez6 levels are elevated in cerebrospinal fluid of patients with inflammatory pain-associated conditions.

BACE1 CSF Chronic pain Inflammatory pain Seizure-related protein 6

Journal

Pain reports
ISSN: 2471-2531
Titre abrégé: Pain Rep
Pays: United States
ID NLM: 101683899

Informations de publication

Date de publication:
Historique:
received: 22 09 2018
revised: 20 12 2018
accepted: 17 01 2019
entrez: 2 5 2019
pubmed: 2 5 2019
medline: 2 5 2019
Statut: epublish

Résumé

Seizure-related protein 6 (Sez6) contributes to chronic pain development as To determine whether this BACE1-shed form of Sez6 can be detected in the cerebrospinal fluid (CSF) and whether Sez6 levels in the CSF are altered in neuropathic pain or chronic inflammatory pain (IP). We analysed the CSF samples collected during surgery from patients with chronic neuropathic pain (n = 8) or IP (n = 33), comparing them to the CSF samples from patients with suspected subarachnoid haemorrhage that was subsequently excluded (nonsurgical group, n = 5). Western blots were used to determine the relative Sez6 levels in the CSF from the different patient and nonsurgical comparison groups. The results show that BACE1-shed Sez6 can be readily detected in the CSF by Western blot and that the levels of Sez6 are significantly higher in the IP group than in the nonsurgical comparison group. The association between elevated Sez6 levels in the CSF and IP is further evidence for persistent alterations in central nervous system activity in chronic IP conditions.

Identifiants

pubmed: 31041421
doi: 10.1097/PR9.0000000000000719
pii: PAINREPORTS-D-18-0082
pmc: PMC6455686
doi:

Types de publication

Journal Article

Langues

eng

Pagination

e719

Subventions

Organisme : NINDS NIH HHS
ID : R01 NS102722
Pays : United States

Déclaration de conflit d'intérêts

Sponsorships or competing interests that may be relevant to content are disclosed at the end of this article.

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Auteurs

Maria Roitman (M)

Department of Anatomy and Neuroscience, The University of Melbourne, Parkville, Victoria, Australia.

Laura E Edgington-Mitchell (LE)

Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria, Australia.
Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Victoria, Australia.
Department of Maxillofacial Surgery, College of Dentistry, New York University, New York, NY, USA.

Jon Mangum (J)

Department of Pharmacology and Therapeutics, The University of Melbourne Parkville, Victoria, Australia.

James Ziogas (J)

Department of Pharmacology and Therapeutics, The University of Melbourne Parkville, Victoria, Australia.

Alexios A Adamides (AA)

Department of Neurosurgery, Royal Melbourne Hospital, Parkville, Victoria, Australia.

Paul Myles (P)

Department of Anaesthesia and Perioperative Medicine, Alfred Hospital, Melbourne, Victoria, Australia.

Hearan Choo-Bunnett (H)

Department of Surgery, College of Physicians and Surgeons, Columbia University, New York, NY, USA.

Nigel W Bunnett (NW)

Department of Surgery, College of Physicians and Surgeons, Columbia University, New York, NY, USA.

Jenny M Gunnersen (JM)

Department of Anatomy and Neuroscience, The University of Melbourne, Parkville, Victoria, Australia.

Classifications MeSH