Multiscale, multispectral fluorescence lifetime imaging using a double-clad fiber.
Journal
Optics letters
ISSN: 1539-4794
Titre abrégé: Opt Lett
Pays: United States
ID NLM: 7708433
Informations de publication
Date de publication:
01 May 2019
01 May 2019
Historique:
entrez:
2
5
2019
pubmed:
2
5
2019
medline:
2
5
2019
Statut:
ppublish
Résumé
Fiber-based imaging of tissue autofluorescence using ultraviolet (UV) excitation is a highly flexible tool used to probe structure and composition. In this Letter, we report, to the best of our knowledge, the first results from a single-fiber imaging system employing a custom double-clad fiber to acquire multispectral fluorescence lifetime images at two distinct spatial resolutions. We characterize the lateral point spread function and fluorescent background of the system and show how enhanced resolution can identify features such as trabeculae in ex vivo murine bone samples.
Identifiants
pubmed: 31042209
pii: 409319
doi: 10.1364/OL.44.002302
pmc: PMC7539568
mid: NIHMS1632490
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2302-2305Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL121068
Pays : United States
Organisme : NIBIB NIH HHS
ID : R03 EB025565
Pays : United States
Références
Appl Opt. 2008 Sep 1;47(25):4560-8
pubmed: 18758526
Phys Med Biol. 2012 Feb 21;57(4):843-65
pubmed: 22290334
Sci Rep. 2017 Aug 21;7(1):8960
pubmed: 28827758
Phys Rev Lett. 2012 Nov 16;109(20):203901
pubmed: 23215488
J Biomed Opt. 2013 Sep;18(9):096001
pubmed: 24002188
Opt Lett. 2017 Oct 1;42(19):3753-3756
pubmed: 28957119
J Biomed Opt. 2018 Mar;23(3):1-9
pubmed: 29512359
Sci Rep. 2019 Feb 4;9(1):1187
pubmed: 30718542
J Biophotonics. 2018 Sep;11(9):e201700391
pubmed: 29781171
Biomed Opt Express. 2015 Feb 25;6(3):987-1002
pubmed: 25798320
Opt Lett. 2008 Oct 15;33(20):2347-9
pubmed: 18923618
Appl Opt. 1981 Feb 15;20(4):701-6
pubmed: 20309181
Tissue Eng Part C Methods. 2012 Sep;18(9):677-87
pubmed: 22439610
Opt Express. 2009 Jan 19;17(2):894-905
pubmed: 19158904
Opt Lett. 2016 Nov 15;41(22):5214-5217
pubmed: 27842096
Photochem Photobiol. 2010 May-Jun;86(3):727-31
pubmed: 20331523
Eur J Histochem. 2014 Dec 12;58(4):2461
pubmed: 25578980
Opt Lett. 2016 Jul 15;41(14):3209-12
pubmed: 27420497