Nanofiber-hydrogel composite-mediated angiogenesis for soft tissue reconstruction.

Animals Cell Movement Cell Polarity Disease Models, Animal Gene Expression Regulation Hydrogels / chemistry Macrophages / pathology Models, Animal Nanofibers / chemistry Neovascularization, Physiologic / genetics Phenotype Rabbits Rats Subcutaneous Tissue / pathology Tissue Engineering

Journal

Science translational medicine
ISSN: 1946-6242
Titre abrégé: Sci Transl Med
Pays: United States
ID NLM: 101505086

Informations de publication

Date de publication:
01 05 2019
Historique:
received: 29 06 2018
accepted: 15 03 2019
entrez: 3 5 2019
pubmed: 3 5 2019
medline: 27 6 2020
Statut: ppublish

Résumé

Soft tissue losses from tumor removal, trauma, aging, and congenital malformation affect millions of people each year. Existing options for soft tissue restoration have several drawbacks: Surgical options such as the use of autologous tissue flaps lead to donor site defects, prosthetic implants are prone to foreign body response leading to fibrosis, and fat grafting and dermal fillers are limited to small-volume defects and only provide transient volume restoration. In addition, large-volume fat grafting and other tissue-engineering attempts are hampered by poor vascular ingrowth. Currently, there are no off-the-shelf materials that can fill the volume lost in soft tissue defects while promoting early angiogenesis. Here, we report a nanofiber-hydrogel composite that addresses these issues. By incorporating interfacial bonding between electrospun poly(ε-caprolactone) fibers and a hyaluronic acid hydrogel network, we generated a composite that mimics the microarchitecture and mechanical properties of soft tissue extracellular matrix. Upon subcutaneous injection in a rat model, this composite permitted infiltration of host macrophages and conditioned them into the pro-regenerative phenotype. By secreting pro-angiogenic cytokines and growth factors, these polarized macrophages enabled gradual remodeling and replacement of the composite with vascularized soft tissue. Such host cell infiltration and angiogenesis were also observed in a rabbit model for repairing a soft tissue defect filled with the composite. This injectable nanofiber-hydrogel composite augments native tissue regenerative responses, thus enabling durable soft tissue restoration outcomes.

Identifiants

DOI: 10.1126/scitranslmed.aau6210 PMID: 31043572
pubmed: 31043572
pii: 11/490/eaau6210
doi: 10.1126/scitranslmed.aau6210
pii:
doi:

Substances chimiques

Hydrogels 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NINDS NIH HHS
ID : R21 NS085714
Pays : United States

Informations de copyright

Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

Auteurs

Xiaowei Li (X)

Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
ORCID: 0000-0003-0714-1140

Brian Cho (B)

Department of Plastic and Reconstructive Surgery, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0001-6706-8390

Russell Martin (R)

Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
ORCID: 0000-0002-6483-6005

Michelle Seu (M)

Department of Plastic and Reconstructive Surgery, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0002-6122-4767

Chi Zhang (C)

Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
ORCID: 0000-0001-8201-3953

Zhengbing Zhou (Z)

Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.

Ji Suk Choi (JS)

Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
ORCID: 0000-0002-7127-1858

Xuesong Jiang (X)

Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
ORCID: 0000-0002-6564-6190

Long Chen (L)

Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
ORCID: 0000-0002-7347-3074

Gurjot Walia (G)

Department of Plastic and Reconstructive Surgery, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
ORCID: 0000-0002-5212-0077

Jerry Yan (J)

Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.

Megan Callanan (M)

Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.

Huanhuan Liu (H)

Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.

Kevin Colbert (K)

Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
ORCID: 0000-0003-1594-5464

Justin Morrissette-McAlmon (J)

Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.

Warren Grayson (W)

Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
ORCID: 0000-0001-6099-6469

Sashank Reddy (S)

Department of Plastic and Reconstructive Surgery, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA. hmao@jhu.edu jmsacks@jhmi.edu sreddy6@jhmi.edu.
ORCID: 0000-0001-6323-3950

Justin M Sacks (JM)

Department of Plastic and Reconstructive Surgery, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA. hmao@jhu.edu jmsacks@jhmi.edu sreddy6@jhmi.edu.

Hai-Quan Mao (HQ)

Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA. hmao@jhu.edu jmsacks@jhmi.edu sreddy6@jhmi.edu.
Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
ORCID: 0000-0002-4262-9988

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Nanofiber-hydrogel composite-mediated...
questionsmedicales.fr › Phénomènes physiologiques › Mouvement cellulaire › Nanofiber-hydrogel composite-mediated...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Polarité de la cellule › Nanofiber-hydrogel composite-mediated...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › Nanofiber-hydrogel composite-mediated...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Nanofiber-hydrogel composite-mediated...
questionsmedicales.fr › Préparations pharmaceutiques › Colloïdes › Gels › Hydrogels › Nanofiber-hydrogel composite-mediated...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Macrophages › Nanofiber-hydrogel composite-mediated...
questionsmedicales.fr › Techniques d'investigation › Modèles animaux › Nanofiber-hydrogel composite-mediated...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanofibres › Nanofiber-hydrogel composite-mediated...
questionsmedicales.fr › Phénomènes physiologiques respiratoires et circulatoires › Phénomènes physiologiques cardiovasculaires › Néovascularisation physiologique › Nanofiber-hydrogel composite-mediated...
questionsmedicales.fr › Phénomènes génétiques › Phénotype › Nanofiber-hydrogel composite-mediated...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Lagomorpha › Lapins › Nanofiber-hydrogel composite-mediated...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Nanofiber-hydrogel composite-mediated...
questionsmedicales.fr › Tissus › Tissu conjonctif › Tissu sous-cutané › Nanofiber-hydrogel composite-mediated...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Ingénierie tissulaire › Nanofiber-hydrogel composite-mediated...