HIV Transcription Is Independent of Mediator Kinases.

Cell Line, Tumor Cyclin-Dependent Kinase 8 / antagonists & inhibitors Cyclin-Dependent Kinases / antagonists & inhibitors HIV-1 / genetics HeLa Cells Humans Jurkat Cells Polycyclic Compounds / pharmacology RNA Interference RNA, Small Interfering / genetics Transcription, Genetic / genetics Virus Activation / drug effects Virus Latency / drug effects
CDK19 CDK8 Cortistatin A HIV transcription Senexin A mediator kinases

Journal

AIDS research and human retroviruses
ISSN: 1931-8405
Titre abrégé: AIDS Res Hum Retroviruses
Pays: United States
ID NLM: 8709376

Informations de publication

Date de publication:
08 2019
Historique:
pubmed: 3 5 2019
medline: 9 7 2020
entrez: 3 5 2019
Statut: ppublish

Résumé

While the roles in HIV transcription of many cyclin-dependent kinases (CDKs) have been well defined, little is known about the impact of mediator kinases (MDKs), CDK8 and CDK19, in this process. Mediator complexes containing CDK8 or CDK19 repress or activate the expression of selected genes. The aim of this study was to investigate the role of MDKs in HIV transcription. siRNA knockdown of both MDKs had no effect on HIV transcription. This result was confirmed using two MDK inhibitors, Cortistatin A (CA) and Senexin A (SnxA). Furthermore, neither CA nor SnxA inhibited viral reactivation in Jurkat cell models of HIV latency. Taken together, these results indicate that MDKs are not required for HIV transcription.

Identifiants

DOI: 10.1089/AID.2019.0039 PMID: 31044597 PMC: PMC6688115
pubmed: 31044597
doi: 10.1089/AID.2019.0039
pmc: PMC6688115
doi:

Substances chimiques

Polycyclic Compounds 0
RNA, Small Interfering 0
cortistatin A 6VG04XOV89
CDK19 protein, human EC 2.7.11.22
CDK8 protein, human EC 2.7.11.22
Cyclin-Dependent Kinase 8 EC 2.7.11.22
Cyclin-Dependent Kinases EC 2.7.11.22

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

710-717

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI049104
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI125104
Pays : United States
Organisme : NIGMS NIH HHS
ID : P50 GM082250
Pays : United States
Organisme : NIAID NIH HHS
ID : P30 AI027763
Pays : United States
Organisme : NIAID NIH HHS
ID : P50 AI150476
Pays : United States

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Auteurs

Daniele C Cary (DC)

Department of Medicine, University of California at San Francisco, San Francisco, California.

Mona Rheinberger (M)

Department of Medicine, University of California at San Francisco, San Francisco, California.

Ajda Rojc (A)

Department of Medicine, University of California at San Francisco, San Francisco, California.

B Matija Peterlin (BM)

Department of Medicine, University of California at San Francisco, San Francisco, California.

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Lignée cellulaire tumorale › HIV Transcription Is Independent of Mediator Kinases.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines nucléaires › Complexe médiateur › Cyclin-Dependent Kinase 8 › HIV Transcription Is Independent of Mediator Kinases.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-Serine-Threonine Kinases › Proline-directed protein kinases › Kinases cyclines-dépendantes › HIV Transcription Is Independent of Mediator Kinases.
questionsmedicales.fr › Virus › Virus à ARN › Retroviridae › Lentivirus › Lentivirus des primates › VIH (Virus de l'Immunodéficience Humaine) › VIH-1 (Virus de l'Immunodéficience Humaine de type 1) › HIV Transcription Is Independent of Mediator Kinases.
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HeLa › HIV Transcription Is Independent of Mediator Kinases.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › HIV Transcription Is Independent of Mediator Kinases.
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Lymphocytes T › Cellules Jurkat › HIV Transcription Is Independent of Mediator Kinases.
questionsmedicales.fr › Composés polycycliques › HIV Transcription Is Independent of Mediator Kinases.
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Interférence par ARN › HIV Transcription Is Independent of Mediator Kinases.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › Petit ARN interférent › HIV Transcription Is Independent of Mediator Kinases.
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Transcription génétique › HIV Transcription Is Independent of Mediator Kinases.
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Réplication virale › Activation virale › HIV Transcription Is Independent of Mediator Kinases.
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Latence virale › HIV Transcription Is Independent of Mediator Kinases.