First Community-Wide, Comparative Cross-Linking Mass Spectrometry Study.
Journal
Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536
Informations de publication
Date de publication:
04 06 2019
04 06 2019
Historique:
pubmed:
3
5
2019
medline:
25
9
2020
entrez:
3
5
2019
Statut:
ppublish
Résumé
The number of publications in the field of chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and to probe protein-protein interactions has increased during the last years. As the technique is now becoming routine for in vitro and in vivo applications in proteomics and structural biology there is a pressing need to define protocols as well as data analysis and reporting formats. Such consensus formats should become accepted in the field and be shown to lead to reproducible results. This first, community-based harmonization study on XL-MS is based on the results of 32 groups participating worldwide. The aim of this paper is to summarize the status quo of XL-MS and to compare and evaluate existing cross-linking strategies. Our study therefore builds the framework for establishing best practice guidelines to conduct cross-linking experiments, perform data analysis, and define reporting formats with the ultimate goal of assisting scientists to generate accurate and reproducible XL-MS results.
Identifiants
pubmed: 31045356
doi: 10.1021/acs.analchem.9b00658
pmc: PMC6625963
mid: EMS83654
doi:
Substances chimiques
Cross-Linking Reagents
0
Serum Albumin, Bovine
27432CM55Q
Types de publication
Journal Article
Multicenter Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
6953-6961Subventions
Organisme : Wellcome Trust
ID : 103139/Z/13/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_U105184326
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : R01 GM074830
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM086688
Pays : United States
Références
Anal Chem. 2000 Jan 15;72(2):267-75
pubmed: 10658319
Proc Natl Acad Sci U S A. 2000 May 23;97(11):5802-6
pubmed: 10811876
Protein Sci. 2000 Aug;9(8):1503-18
pubmed: 10975572
Biochemistry. 2001 Jul 3;40(26):7903-13
pubmed: 11425319
Anal Chem. 2005 Jan 1;77(1):311-8
pubmed: 15623310
Nat Biotechnol. 2007 Aug;25(8):887-93
pubmed: 17687369
Nat Methods. 2009 Jun;6(6):423-30
pubmed: 19448641
Nat Biotechnol. 2009 Jul;27(7):633-41
pubmed: 19561596
Mol Cell Proteomics. 2011 Jan;10(1):R110.000133
pubmed: 20716697
Nat Methods. 2014 Feb;11(2):149-55
pubmed: 24317253
Nat Protoc. 2014 Jan;9(1):120-37
pubmed: 24356771
Protein Sci. 2014 Jun;23(6):747-59
pubmed: 24639379
Nat Protoc. 2014 Sep;9(9):2224-36
pubmed: 25144272
Mol Cell Proteomics. 2015 Sep;14(9):2357-74
pubmed: 25693799
Nucleic Acids Res. 2016 Jan 4;44(D1):D447-56
pubmed: 26527722
Nat Biotechnol. 2016 Nov;34(11):1130-1136
pubmed: 27701404
Nucleic Acids Res. 2017 Jan 4;45(D1):D1100-D1106
pubmed: 27924013
J Proteomics. 2017 Jan 30;152:138-149
pubmed: 27989941
Mol Cell Proteomics. 2017 Jul;16(7):1275-1285
pubmed: 28515314
Nat Commun. 2017 May 19;8:15473
pubmed: 28524877
Nat Commun. 2017 Aug 21;8(1):291
pubmed: 28827567
J Proteome Res. 2017 Dec 1;16(12):4288-4298
pubmed: 28849660
Nat Protoc. 2018 Mar;13(3):431-458
pubmed: 29388937
Nat Protoc. 2018 Mar;13(3):478-494
pubmed: 29419816
Structure. 2018 Jun 5;26(6):894-904.e2
pubmed: 29657133
Nat Protoc. 2018 Dec;13(12):2864-2889
pubmed: 30382245
Nat Protoc. 2018 Dec;13(12):2964-2990
pubmed: 30446747
Nat Protoc. 2019 Jan;14(1):171-201
pubmed: 30559374