Regulation of Plasmodium sporozoite motility by formulation components.
Live in vitro imaging
Motility regulation
Plasmodium berghei
Vaccine formulation
Journal
Malaria journal
ISSN: 1475-2875
Titre abrégé: Malar J
Pays: England
ID NLM: 101139802
Informations de publication
Date de publication:
02 May 2019
02 May 2019
Historique:
received:
18
01
2019
accepted:
25
04
2019
entrez:
4
5
2019
pubmed:
3
5
2019
medline:
27
6
2019
Statut:
epublish
Résumé
The protective efficacy of the most promising malaria whole-parasite based vaccine candidates critically depends on the parasite's potential to migrate in the human host. Key components of the parasite motility machinery (e.g. adhesive proteins, actin/myosin-based motor, geometrical properties) have been identified, however the regulation of this machinery is an unknown process. In vitro microscopic live imaging of parasites in different formulations was performed and analysed, with the quantitative analysis software SMOOT SMOOT Based on the findings can be concluded that a complex interplay of albumin, glucose and certain salts and amino acids regulates parasite motility. Insights in parasite motility regulation by supplements in solution potentially provide a way to optimize the whole-parasite malaria vaccine formulation.
Sections du résumé
BACKGROUND
BACKGROUND
The protective efficacy of the most promising malaria whole-parasite based vaccine candidates critically depends on the parasite's potential to migrate in the human host. Key components of the parasite motility machinery (e.g. adhesive proteins, actin/myosin-based motor, geometrical properties) have been identified, however the regulation of this machinery is an unknown process.
METHODS
METHODS
In vitro microscopic live imaging of parasites in different formulations was performed and analysed, with the quantitative analysis software SMOOT
RESULTS
RESULTS
SMOOT
CONCLUSIONS
CONCLUSIONS
Based on the findings can be concluded that a complex interplay of albumin, glucose and certain salts and amino acids regulates parasite motility. Insights in parasite motility regulation by supplements in solution potentially provide a way to optimize the whole-parasite malaria vaccine formulation.
Identifiants
pubmed: 31046772
doi: 10.1186/s12936-019-2794-y
pii: 10.1186/s12936-019-2794-y
pmc: PMC6498664
doi:
Substances chimiques
Albumins
0
Culture Media
0
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
155Subventions
Organisme : FP7 Ideas: European Research Council
ID : 2012-306890
Organisme : ZonMw
ID : VENI 016.156.076
Organisme : Leids Universitair Medisch Centrum
ID : Gisela Thier fellowship
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