Mechanism of cognate sequence discrimination by the ETS-family transcription factor ETS-1.
DNA binding protein
DNA sequence motif
DNA-binding domain
ETS proto-oncogene 1 transcription factor (ETS1)
ETS transcription factor family
allosteric regulation
developmental factor
low-affinity DNA binding
molecular dynamics
suboptimization
transcription enhancer
transcription factor
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
21 06 2019
21 06 2019
Historique:
received:
01
02
2019
revised:
01
05
2019
pubmed:
3
5
2019
medline:
4
3
2020
entrez:
4
5
2019
Statut:
ppublish
Résumé
Functional evidence increasingly implicates low-affinity DNA recognition by transcription factors as a general mechanism for the spatiotemporal control of developmental genes. Although the DNA sequence requirements for affinity are well-defined, the dynamic mechanisms that execute cognate recognition are much less resolved. To address this gap, here we examined ETS1, a paradigm developmental transcription factor, as a model for which cognate discrimination remains enigmatic. Using molecular dynamics simulations, we interrogated the DNA-binding domain of murine ETS1 alone and when bound to high-and low-affinity cognate sites or to nonspecific DNA. The results of our analyses revealed collective backbone and side-chain motions that distinguished cognate
Identifiants
pubmed: 31048376
pii: S0021-9258(20)34700-1
doi: 10.1074/jbc.RA119.007866
pmc: PMC6597803
doi:
Substances chimiques
Ets1 protein, mouse
0
Proto-Oncogene Protein c-ets-1
0
DNA
9007-49-2
Banques de données
PDB
['1K79', '1R36', '3MFK', '1MDM']
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
9666-9678Informations de copyright
© 2019 Huang et al.
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