Measuring Intracellular Viscosity in Conditions of Hypergravity.
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
21 05 2019
21 05 2019
Historique:
received:
12
11
2018
revised:
28
02
2019
accepted:
19
03
2019
pubmed:
6
5
2019
medline:
27
6
2020
entrez:
5
5
2019
Statut:
ppublish
Résumé
Gravity-sensitive cellular responses are regularly observed in both specialized and nonspecialized cells. One potential mechanism for this sensitivity is a changing viscosity of the intracellular organelles. Here, we report a novel, to our knowledge, viscosity-sensitive molecular rotor based on mesosubstituted boron-dipyrrin used to investigate the response of viscosity of cellular membranes to hypergravity conditions created at the large diameter centrifuge at the European Space Agency Technology Centre. Mouse osteoblastic (MC3T3-E1) and endothelial (human umbilical vein endothelial cell) cell lines were tested, and an increase in viscosity was found with increasing hypergravity loading. This response is thought to be primarily biologically driven, with the potential for a small, instantaneous physical mechanism also contributing to the observed effect. This work provides the first, to our knowledge, quantitative data for cellular viscosity changes under hypergravity, up to 15 × g.
Identifiants
pubmed: 31053255
pii: S0006-3495(19)30298-X
doi: 10.1016/j.bpj.2019.03.038
pmc: PMC6531836
pii:
doi:
Substances chimiques
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
0
Boron Compounds
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1984-1993Informations de copyright
Copyright © 2019. Published by Elsevier Inc.
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