LPS-squalene interaction on D-galactose intestinal absorption.
Animals
Biological Transport
/ drug effects
Caco-2 Cells
Galactose
/ metabolism
Humans
Intestinal Absorption
/ drug effects
Intestinal Mucosa
/ drug effects
Lipopolysaccharides
/ adverse effects
Myosin-Light-Chain Kinase
/ metabolism
Rabbits
Sodium-Glucose Transporter 1
/ metabolism
Squalene
/ pharmacology
Intestinal absorption
LPS
MLCK
RELM-β
SGLT1
Squalene
Journal
Journal of physiology and biochemistry
ISSN: 1877-8755
Titre abrégé: J Physiol Biochem
Pays: Spain
ID NLM: 9812509
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
23
10
2018
accepted:
17
04
2019
pubmed:
6
5
2019
medline:
23
2
2020
entrez:
5
5
2019
Statut:
ppublish
Résumé
The dynamic and complex interactions between enteric pathogens and the intestinal epithelium often lead to disturbances in the intestinal barrier, altered fluid, electrolyte, and nutrient transport and can produce an inflammatory response. Lipopolysaccharide (LPS) is a complex polymer forming part of the outer membrane of Gram-negative bacteria. On the other hand, squalene is a triterpene present in high levels in the extra-virgin olive oil that has beneficial effects against several diseases and it has also anti-oxidant and anti-inflammatory properties. The aim of this work was to study whether the squalene could eliminate the LPS effect on D-galactose intestinal absorption in rabbits and Caco-2 cells. The results have shown that squalene reduced the effects of LPS on sugar absorption. High LPS doses increased D-galactose uptake through via paracellular but also decreased the active sugar transport because the SGLT1 levels were diminished. However, the endotoxin effect on the paracellular way seemed to be more important than on the transcellular route. At the same time, an increased in RELM-β expression was observed. This event could be related to inflammation and cause a decrease in SGLT1 levels. In addition, MLCK protein is also increased by LPS which could lead to an increase in sugar transport through tight junctions. At low doses, the LPS could inhibit SGLT1 intrinsic activity. Bioinformatic studies by docking confirm the interaction between LPS-squalene as well as occur through MLCK and SGLT-1 proteins.
Identifiants
pubmed: 31054079
doi: 10.1007/s13105-019-00682-8
pii: 10.1007/s13105-019-00682-8
doi:
Substances chimiques
Lipopolysaccharides
0
Sodium-Glucose Transporter 1
0
Squalene
7QWM220FJH
Myosin-Light-Chain Kinase
EC 2.7.11.18
Galactose
X2RN3Q8DNE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
329-340Références
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