Troponin 1 of human filarial parasite Brugia malayi: cDNA cloning, expression, purification, and its immunoprophylactic potential.
Animals
Antibodies, Protozoan
/ blood
Antibody-Dependent Cell Cytotoxicity
/ immunology
Brugia malayi
/ genetics
Cloning, Molecular
Cytokines
/ blood
DNA, Complementary
/ metabolism
Elephantiasis, Filarial
/ immunology
Humans
Immunoglobulin E
/ blood
Immunoglobulin G
/ blood
Macrophages, Peritoneal
/ immunology
Mice
Mice, Inbred BALB C
Parasite Load
Th1 Cells
/ immunology
Th2 Cells
/ immunology
Troponin I
/ genetics
Vaccination
ADCC
Brugia malayi
CD19+
CD3+
CD4+
CD8+
Immunoprophylaxis
MHC class-I
MHC class-II
TLRs
Th1 and Th2 responses
Troponin 1
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
05
07
2018
accepted:
03
04
2019
pubmed:
6
5
2019
medline:
13
7
2019
entrez:
6
5
2019
Statut:
ppublish
Résumé
In the search for immunoprophylactics for the control of human lymphatic filariasis, we recently identified troponin 1 (Tn1) in Brugia malayi adult worms. The present study reports the cloning and expression of the B. malayi Tn1 (Tn1bm), its immunoprophylactic efficacy against B. malayi infection, and the immunological responses of the host. The Tn1bm gene was cloned (Acc no. JF912447) and expressed, and the purified recombinant Tn1bm (rTn1bm) presented a single ~ 27 kDa band. Parasite load in rTn1bm-immunized BALB/c mice challenged with B. malayi infective larvae (L
Identifiants
pubmed: 31055672
doi: 10.1007/s00436-019-06316-8
pii: 10.1007/s00436-019-06316-8
doi:
Substances chimiques
Antibodies, Protozoan
0
Cytokines
0
DNA, Complementary
0
Immunoglobulin G
0
Troponin I
0
Immunoglobulin E
37341-29-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1849-1863Références
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