Transcriptional Profiling of Non-Human Primate Lymphoid Organ Responses to Total-Body Irradiation.
Journal
Radiation research
ISSN: 1938-5404
Titre abrégé: Radiat Res
Pays: United States
ID NLM: 0401245
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
pubmed:
7
5
2019
medline:
19
10
2019
entrez:
7
5
2019
Statut:
ppublish
Résumé
The global threat of exposure to radiation and its subsequent outcomes require the development of effective strategies to mitigate immune cell injury. In this study we explored transcriptional and immunophenotypic characteristics of lymphoid organs of a non-human primate model after total-body irradiation (TBI). Fifteen middle-aged adult, ovariectomized, female cynomolgus macaques received a single dose of 0, 2 or 5 Gy gamma radiation. Thymus, spleen and lymph node from three controls and 2 Gy (n = 2) and 5 Gy (n = 2) exposed animals were assessed for molecular responses to TBI through microarray-based transcriptional profiling at day 5 postirradiation, and cellular changes through immunohistochemical (IHC) characterization of markers for B and T lymphocytes and macrophages across all 15 animals at time points up to 6 months postirradiation. Irradiated macaques developed acute hematopoietic syndrome. Analysis of array data at day 5 postirradiation identified transcripts with ≥2-fold difference from control and a false discovery rate (FDR) of
Identifiants
pubmed: 31059377
pii: 10.1667/RR15100.1
doi: 10.1667/RR15100.1
pmc: PMC6699496
mid: NIHMS1040557
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
40-52Subventions
Organisme : NIH HHS
ID : T32 OD010957
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI067798
Pays : United States
Organisme : NIAID NIH HHS
ID : UC6 AI058607
Pays : United States
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