Meta-transcriptomic analysis reveals a new subtype of genotype 3 avian hepatitis E virus in chicken flocks with high mortality in Guangdong, China.
Avian hepatitis E virus
High mortality
Meta-transcriptomics analysis
New subtype
Journal
BMC veterinary research
ISSN: 1746-6148
Titre abrégé: BMC Vet Res
Pays: England
ID NLM: 101249759
Informations de publication
Date de publication:
06 May 2019
06 May 2019
Historique:
received:
05
11
2018
accepted:
25
04
2019
entrez:
8
5
2019
pubmed:
8
5
2019
medline:
22
5
2019
Statut:
epublish
Résumé
Hepatitis E virus (HEV) is one of most important zoonotic viruses, and it can infect a wide range of host species. Avian HEV has been identified as the aetiological agent of big liver and spleen disease or hepatitis-splenomegaly syndrome in chickens. HEV infection is common among chicken flocks in China, and there are currently no practical measures for preventing the spread of the disease. The predominant avian HEV genotype circulating in China have been identified as genotype 3 strains, although some novel genotypes have also been identified from chicken flocks in China. In this study, we used a meta-transcriptomics approach to identify a new subtype of genotype 3 avian HEV in broiler chickens at a poultry farm located in Shenzhen, Guangdong Province, China. The complete genome sequence of the avian HEV, designated CaHEV-GDSZ01, is 6655-nt long, including a 5' UTR of 24 nt and a 3' UTR of 125 nt (excluding the poly(A) tail), and contains three open reading frames (ORFs). Sequence analysis indicated that the complete ORF1 (4599 nt/1532 aa), ORF2 (1821 nt/606 aa) and ORF3 (264 nt/87 aa) of CaHEV-GDSZ01 share the highest nucleotide sequence identity (85.8, 86.7 and 95.8%, respectively) with the corresponding ORFs of genotype 3 avian HEV. Phylogenetic analyses further demonstrated that the avian HEV identified in this study is a new subtype of genotype 3 avian HEV. Our results demonstrate that a new subtype of genotype 3 avian HEV is endemic in Guangdong, China, and could cause high mortality in infected chickens. This study also provides full genomic data for better understanding the evolutionary relationships of avian HEV circulating in China. Altogether, the results presented in this study suggest that more attention should be paid to avian HEV and its potential disease manifestation.
Sections du résumé
BACKGROUND
BACKGROUND
Hepatitis E virus (HEV) is one of most important zoonotic viruses, and it can infect a wide range of host species. Avian HEV has been identified as the aetiological agent of big liver and spleen disease or hepatitis-splenomegaly syndrome in chickens. HEV infection is common among chicken flocks in China, and there are currently no practical measures for preventing the spread of the disease. The predominant avian HEV genotype circulating in China have been identified as genotype 3 strains, although some novel genotypes have also been identified from chicken flocks in China.
RESULTS
RESULTS
In this study, we used a meta-transcriptomics approach to identify a new subtype of genotype 3 avian HEV in broiler chickens at a poultry farm located in Shenzhen, Guangdong Province, China. The complete genome sequence of the avian HEV, designated CaHEV-GDSZ01, is 6655-nt long, including a 5' UTR of 24 nt and a 3' UTR of 125 nt (excluding the poly(A) tail), and contains three open reading frames (ORFs). Sequence analysis indicated that the complete ORF1 (4599 nt/1532 aa), ORF2 (1821 nt/606 aa) and ORF3 (264 nt/87 aa) of CaHEV-GDSZ01 share the highest nucleotide sequence identity (85.8, 86.7 and 95.8%, respectively) with the corresponding ORFs of genotype 3 avian HEV. Phylogenetic analyses further demonstrated that the avian HEV identified in this study is a new subtype of genotype 3 avian HEV.
CONCLUSIONS
CONCLUSIONS
Our results demonstrate that a new subtype of genotype 3 avian HEV is endemic in Guangdong, China, and could cause high mortality in infected chickens. This study also provides full genomic data for better understanding the evolutionary relationships of avian HEV circulating in China. Altogether, the results presented in this study suggest that more attention should be paid to avian HEV and its potential disease manifestation.
Identifiants
pubmed: 31060564
doi: 10.1186/s12917-019-1884-y
pii: 10.1186/s12917-019-1884-y
pmc: PMC6503432
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
131Subventions
Organisme : Key Laboratory for Preventive Research of Emerging Animal Diseases in Foshan University
ID : KLPREAD201801-09
Organisme : Key Laboratory for Preventive Research of Emerging Animal Diseases in Foshan University
ID : KLPREAD201801-08
Organisme : National Plan for Science, Technology and Innovation (SA)
ID : 2012A020100001
Organisme : Department of Education of Guangdong Province
ID : 2014KTSPT037
Références
J Biol Chem. 2002 Jun 21;277(25):22759-67
pubmed: 11934888
J Gen Virol. 2002 Sep;83(Pt 9):2201-9
pubmed: 12185274
Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8259-63
pubmed: 1518855
J Virol. 2007 Mar;81(6):3018-26
pubmed: 17202216
Mol Biol Evol. 2008 Jul;25(7):1253-6
pubmed: 18397919
J Gen Virol. 2009 Apr;90(Pt 4):863-73
pubmed: 19264623
Virology. 1991 Nov;185(1):120-31
pubmed: 1926770
J Viral Hepat. 2010 Mar;17(3):153-61
pubmed: 20040046
Syst Biol. 2010 May;59(3):307-21
pubmed: 20525638
Emerg Infect Dis. 2010 Sep;16(9):1469-72
pubmed: 20735936
Nat Biotechnol. 2011 May 15;29(7):644-52
pubmed: 21572440
J Viral Hepat. 2011 Oct;18(10):e583-90
pubmed: 21914080
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Brief Bioinform. 2013 Mar;14(2):178-92
pubmed: 22517427
Virus Genes. 2012 Oct;45(2):237-45
pubmed: 22639103
Emerg Infect Dis. 2012 Aug;18(8):1365-8
pubmed: 22840214
Avian Pathol. 2012 Oct;41(5):459-63
pubmed: 22967203
J Virol Methods. 2013 Jan;187(1):32-6
pubmed: 23000753
J Med Virol. 2013 Jun;85(6):1037-45
pubmed: 23588729
Mol Biol Evol. 2013 Dec;30(12):2725-9
pubmed: 24132122
Emerg Infect Dis. 2014 Jan;20(1):149-51
pubmed: 24378180
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
J Gen Virol. 2014 Oct;95(Pt 10):2223-32
pubmed: 24989172
Virus Genes. 2016 Oct;52(5):738-42
pubmed: 27164843
Avian Dis. 2016 Sep;60(3):698-704
pubmed: 27610734
Viruses. 2016 Oct 03;8(10):
pubmed: 27706110
BMC Vet Res. 2016 Nov 22;12(1):261
pubmed: 27876045
Ticks Tick Borne Dis. 2017 Jun;8(4):574-580
pubmed: 28411028
Int J Mol Sci. 2017 Apr 20;18(4):null
pubmed: 28425927
Vet Microbiol. 2017 May;203:174-180
pubmed: 28619140
J Virol. 2017 Aug 10;91(17):
pubmed: 28637756
Sci Rep. 2017 Sep 7;7(1):10870
pubmed: 28883464
Microb Pathog. 2018 Jun;119:103-108
pubmed: 29621564
Emerg Microbes Infect. 2018 May 2;7(1):76
pubmed: 29717131
Transbound Emerg Dis. 2018 Dec;65(6):2017-2026
pubmed: 30086212
Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9860-5
pubmed: 9275216