Identification and characterization of a secreted M28 aminopeptidase protein in Acanthamoeba.
Acanthamoeba
CRISPR
Complement protein
M28AP
Secreted protein
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
12
02
2019
accepted:
18
04
2019
pubmed:
9
5
2019
medline:
13
7
2019
entrez:
9
5
2019
Statut:
ppublish
Résumé
Acanthamoeba is a free-living pathogenic protozoan that is distributed in different environmental reservoirs, including lakes and soil. Pathogenic Acanthamoeba can cause severe human diseases, such as blinding keratitis and granulomatous encephalitis. Therefore, it is important to understand the pathogenic relationship between humans and Acanthamoeba. By comparison of systemic analysis results for Acanthamoeba isolates, we identified a novel secreted protein of Acanthamoeba, an M28 aminopeptidase (M28AP), which targets of the human innate immune defense. We investigated the molecular functions and characteristics of the M28AP protein by anti-M28 antibodies and a M28AP mutant strain generated by the CRISPR/Cas9 system. Human complement proteins such as C3b and iC3b were degraded by Acanthamoeba M28AP. We believe that M28AP is an important factor in human innate immunity. This study provides new insight for the development of more efficient medicines to treat Acanthamoeba infection.
Identifiants
pubmed: 31065830
doi: 10.1007/s00436-019-06332-8
pii: 10.1007/s00436-019-06332-8
doi:
Substances chimiques
Complement C3
0
Protozoan Proteins
0
Soil
0
Aminopeptidases
EC 3.4.11.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1865-1874Références
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