Extracellular Matrix Geometry and Initial Adhesive Position Determine Stress Fiber Network Organization during Cell Spreading.
Actin Cytoskeleton
/ drug effects
Cell Adhesion
/ drug effects
Cell Line, Tumor
Cell Movement
/ drug effects
Collagen
/ metabolism
Computer Simulation
Extracellular Matrix
/ drug effects
Half-Life
Heterocyclic Compounds, 4 or More Rings
/ pharmacology
Humans
Kinetics
Models, Biological
Pseudopodia
/ drug effects
Stress Fibers
/ drug effects
Time Factors
actin cytoskeleton
cell memory
cell migration
cell shape
cell spreading
cell-matrix adhesion
cellular Potts model
mathematical modeling
mechanobiology
stress fibers
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
07 05 2019
07 05 2019
Historique:
received:
14
05
2018
revised:
25
02
2019
accepted:
05
04
2019
entrez:
9
5
2019
pubmed:
9
5
2019
medline:
2
7
2020
Statut:
ppublish
Résumé
Three-dimensional matrices often contain highly structured adhesive tracks that require cells to turn corners and bridge non-adhesive areas. Here, we investigate these complex processes using micropatterned cell adhesive frames. Spreading kinetics on these matrices depend strongly on initial adhesive position and are predicted by a cellular Potts model (CPM), which reflects a balance between adhesion and intracellular tension. As cells spread, new stress fibers (SFs) assemble periodically and parallel to the leading edge, with spatial intervals of ∼2.5 μm, temporal intervals of ∼15 min, and characteristic lifetimes of ∼50 min. By incorporating these rules into the CPM, we can successfully predict SF network architecture. Moreover, we observe broadly similar behavior when we culture cells on arrays of discrete collagen fibers. Our findings show that ECM geometry and initial cell position strongly determine cell spreading and that cells encode a memory of their spreading history through SF network organization.
Identifiants
pubmed: 31067472
pii: S2211-1247(19)30500-5
doi: 10.1016/j.celrep.2019.04.035
pmc: PMC6530591
mid: NIHMS1528924
pii:
doi:
Substances chimiques
Heterocyclic Compounds, 4 or More Rings
0
blebbistatin
20WC4J7CQ6
Collagen
9007-34-5
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1897-1909.e4Subventions
Organisme : NIGMS NIH HHS
ID : F31 GM119329
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM122375
Pays : United States
Organisme : NIBIB NIH HHS
ID : R21 EB025017
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
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