Proteomic characterisation of leech microglia extracellular vesicles (EVs): comparison between differential ultracentrifugation and Optiprep™ density gradient isolation.
Hirudo medicinalis
Optiprep™
extracellular vesicles
microglia
neurite outgrowth
protein content
ultracentrifugation
Journal
Journal of extracellular vesicles
ISSN: 2001-3078
Titre abrégé: J Extracell Vesicles
Pays: United States
ID NLM: 101610479
Informations de publication
Date de publication:
2019
2019
Historique:
received:
27
07
2018
revised:
29
03
2019
accepted:
01
04
2019
entrez:
10
5
2019
pubmed:
10
5
2019
medline:
10
5
2019
Statut:
epublish
Résumé
In Mammals, microglial cells are considered as the resident immune cells in central nervous system (CNS). Many studies demonstrated that, after injury, these cells are activated and recruited at the lesion site. Leech microglia present a similar pattern of microglial activation and migration upon experimental lesion of CNS. This activation is associated with the release of a large amount of extracellular vesicles (EVs). We collected EVs released by microglia primary culture and compared two different protocols of isolation: one with differential ultracentrifugation (UC) and one using an additional Optiprep
Identifiants
pubmed: 31069026
doi: 10.1080/20013078.2019.1603048
pii: 1603048
pmc: PMC6493217
doi:
Types de publication
Journal Article
Langues
eng
Pagination
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