Electrical synapses between mushroom body neurons are critical for consolidated memory retrieval in Drosophila.
Animals
Brain
/ metabolism
Carbenoxolone
/ pharmacology
Connexins
/ genetics
Drosophila Proteins
/ genetics
Drosophila melanogaster
/ genetics
Electrical Synapses
/ metabolism
Gap Junctions
/ metabolism
Memory
/ physiology
Mushroom Bodies
/ metabolism
Neurons
/ metabolism
Odorants
Smell
/ genetics
Synaptic Transmission
/ physiology
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
18
01
2019
accepted:
23
04
2019
revised:
21
05
2019
pubmed:
10
5
2019
medline:
27
11
2019
entrez:
10
5
2019
Statut:
epublish
Résumé
Electrical synapses between neurons, also known as gap junctions, are direct cell membrane channels between adjacent neurons. Gap junctions play a role in the synchronization of neuronal network activity; however, their involvement in cognition has not been well characterized. Three-hour olfactory associative memory in Drosophila has two components: consolidated anesthesia-resistant memory (ARM) and labile anesthesia-sensitive memory (ASM). Here, we show that knockdown of the gap junction gene innexin5 (inx5) in mushroom body (MB) neurons disrupted ARM, while leaving ASM intact. Whole-mount brain immunohistochemistry indicated that INX5 protein was preferentially expressed in the somas, calyxes, and lobes regions of the MB neurons. Adult-stage-specific knockdown of inx5 in αβ neurons disrupted ARM, suggesting a specific requirement of INX5 in αβ neurons for ARM formation. Hyperpolarization of αβ neurons during memory retrieval by expressing an engineered halorhodopsin (eNpHR) also disrupted ARM. Administration of the gap junction blocker carbenoxolone (CBX) reduced the proportion of odor responsive αβ neurons to the training odor 3 hours after training. Finally, the α-branch-specific 3-hour ARM-specific memory trace was also diminished with CBX treatment and in inx5 knockdown flies. Altogether, our results suggest INX5 gap junction channels in αβ neurons for ARM retrieval and also provide a more detailed neuronal mechanism for consolidated memory in Drosophila.
Identifiants
pubmed: 31071084
doi: 10.1371/journal.pgen.1008153
pii: PGENETICS-D-19-00086
pmc: PMC6529013
doi:
Substances chimiques
Connexins
0
Drosophila Proteins
0
Carbenoxolone
MM6384NG73
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008153Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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